L-selectin expression on thymic emigrants defines two distinct tissue-migration pathways
L-selectin expression on thymic emigrants defines two distinct tissue-migration pathways
复制标题
DOI：
10.1046/j.1365-2567.1999.00882.x
复制
发表时间：
1999-11-01
期刊：
影响因子：
6.4
通讯作者：
Cahill, RNP
中科院分区：
文献类型：
--
作者：
Holder, JE;Kimpton, WG;Cahill, RNP
我们研究了新生羔羊血液、脾脏和淋巴结（LN）中近期胸腺移行细胞的外观和表型。用异硫氰酸荧光素（FITC）原位标记胸腺细胞，检测标记后24小时淋巴结归巢受体L-选择素在近期胸腺移出者的ap和γ δ亚群上的表达。与淋巴结相比，从胸腺输出到脾脏的CD 4（+）、CD 8（+）和γ δ T细胞受体（TCR+）细胞的比例有显著差异。脾脏富含CD 8（+）和γ δ TCR+的移行细胞，而LN富含CD 4（+）的移行细胞。归巢至脾脏和归巢至淋巴结的移行细胞L-选择素的表达也有显著差异。白色淋巴结炎患者胸腺中L-选择素阳性细胞最多，脾脏中L-选择素阳性细胞较少。大量CD 8（+）L-选择素（-）和γ δ TCR+ L-选择素（-）胸腺迁移物归巢至脾脏的存在，提高了独特归巢受体特异性支持T细胞不同于淋巴结向脾脏迁移的可能性。
We have studied the appearance and phenotype of recent thymic emigrants in blood, spleen and lymph nodes (LN) of neonatal lambs. Using in situ labelling of thymocytes with fluoroscein isothiocyanate (FITC), we examined the expression of the LN homing receptor L-selectin on ap and gamma delta subsets of recent thymic emigrants 24 hr after labelling, There were marked differences in the proportions of CD4(+), CD8(+) and gamma delta T-cell receptor (TCR+) cells exported from the thymus to spleen compared to lymph nodes. Spleen was enriched in CD8(+) and gamma delta TCR+ emigrants while LN were enriched in CD4(+) emigrants. There were also marked differences in the expression of L-selectin by emigrants homing to spleen compared with those homing to lymph nodes. White the majority of thymic emigrants in LN expressed L-selectin, considerably fewer emigrants in spleen were L-selectin(+). The presence of large numbers of CD8(+) L-selectin(-) and gamma delta TCR+ L-selectin(-) thymic emigrants homing to spleen raises the possibility that unique homing receptor specificities underpin the migration of T cells to spleen as distinct from lymph nodes.