Studies on rice seed quality through analysis of a large-scale T-DNA insertion population
Studies on rice seed quality through analysis of a large-scale T-DNA insertion population
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DOI：
10.1038/cr.2009.15
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发表时间：
2009-03-01
期刊：
影响因子：
44.1
通讯作者：
Xue, Hong-Wei
中科院分区：
文献类型：
--
作者：
Fu, Fang-Fang;Ye, Rui;Xue, Hong-Wei
为了系统研究水稻种子质量控制，构建了一个包含63 000多个独立转基因株系和8 840个侧翼序列标签（FSTs）的水稻T-DNA插入群体。全基因组FST分布分析表明，T-DNA插入与表达基因呈正相关，而与转座因子和小RNA呈负相关。此外，回收的T-DNA优先位于表达基因的非翻译区。通过多代种植和抗性筛选，获得11 000多个推定纯合株系，并对其中约一半株系的淀粉、直链淀粉、蛋白质和脂肪含量进行无损近红外光谱测定，鉴定出551个具有单一或多个种子品质参数改变的突变体。对相应FST的分析表明，参与多种功能的基因，包括代谢过程和转录调控，都参与其中，这表明种子质量受到复杂网络的调控。
A rice (Oryza sativa) T-DNA insertion population, which included more than 63 000 independent transgenic lines and 8 840 identified flanking sequence tags (FSTs) that were mapped onto the rice genome, was developed to systemically study the rice seed quality control. Genome-wide analysis of the FST distribution showed that T-DNA insertions were positively correlated with expressed genes, but negatively with transposable elements and small RNAs. In addition, the recovered T-DNAs were preferentially located at the untranslated region of the expressed genes. More than 11 000 putative homozygous lines were obtained through multi-generations of planting and resistance screening, and measurement of seed quality of around half of them, including the contents of starch, amylose, protein and fat, with a nondestructive near-infrared spectroscopy method, identified 551 mutants with unique or multiple altered parameters of seed quality. Analysis of the corresponding FSTs showed that genes participating in diverse functions, including metabolic processes and transcriptional regulation, were involved, indicating that seed quality is regulated by a complex network.