Evaluation of HSPA2 in fertile and infertile individuals
Evaluation of HSPA2 in fertile and infertile individuals
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DOI：
10.1111/j.1439-0272.2012.01315.x
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发表时间：
2013-02-01
期刊：
影响因子：
2.4
通讯作者：
Nasr-Esfahani, M. -H.
中科院分区：
文献类型：
--
作者：
Motiei, M.;Tavalaee, M.;Nasr-Esfahani, M. -H.
热休克蛋白A2（Heat-shockproteinA 2，HSPA 2）是热休克蛋白70家族中的一个睾丸特异性成员，与精子的成熟度、功能和生育力密切相关。本研究的目的是比较表达的HSPA 2在生育和不育的个人使用最近上市的高度纯化的多克隆抗体。因此，在分析了49例男性因素不育患者和47例正常生育者精液中的精子密度、活力和形态后，我们通过显微镜荧光、流式细胞术、Western blot和RT-PCR检测了HSPA 2。我们观察到较高比例的精子表达HSPA 2在前部和赤道区的生育比不育的个人显微镜荧光。通过流式细胞术在两种条件下（固定透化和获能）表达HSPA 2的精子百分比显示，固定透化和获能样品中HSPA 2阳性精子的百分比在可育和不育个体之间存在显著差异。精液样本中HSPA 2的Western印迹分析显示，在可育和不育人群中存在高度变异。RT-PCR结果显示，HSPA 2在生育组的表达高于不育组，但差异不显著。根据这些结果，我们认为，HSPA 2的表达是异质性表达的不同部分的固定透化精子和其表达是显着较高的生育能力相比，不育的个人。这种蛋白质的表面表达显着增加获能后，在可育和不育的个人。HSPA 2的表达与精子密度和形态显著相关。因此，异常的HSPA 2表达可能在获能和受精过程中起重要作用。
Heat-shock protein A2 (HSPA2) is a testis-specific member of the HSP70 family known to correlate with sperm maturity, function and fertility. The aim of this study was to compare expression of HSPA2 in fertile and infertile individuals using a recently marketed highly purified polyclonal antibody. Thus, after analysing sperm concentration, motility and morphology in semen sample of 49 individuals with male factor infertility and 47 fertile individuals according to World Health Organization guidelines, we evaluated HSPA2 by microscope florescence, flow cytometry, Western blot and RT-PCR. We observed higher percentage of sperm expressing HSPA2 in anterior and equatorial regions in fertile than infertile individuals by microscope fluorescence. Percentage of sperm expressing HSPA2 in two conditions (fixed permeabilised and capacitated) by flow cytometry showed that percentage of HSPA2-positive sperm in fixed permeabilised and also capacitated samples was significantly different between fertile and infertile individuals. Western blot analysis of HSPA2 in semen samples revealed high variation within the fertile and infertile population. The results of RT-PCR revealed higher expression of HSPA2 in the fertile compared to infertile individuals, but this difference was not significant. According to the results, we suggest that HSPA2 expression is heterogeneously expressed on different part of fixed permeabilised sperm and its expression is significantly higher in fertile compared to infertile individuals. The surface expression of this protein significantly increases following capacitation in both fertile and infertile individuals. HSPA2 expression significantly correlates with sperm concentration and morphology. Therefore, aberrant HSPA2 expression may play an important role in capacitation and fertilisation processes.