FGF treatment of host embryos injected with ES cells increases rates of chimaerism.
FGF treatment of host embryos injected with ES cells increases rates of chimaerism.
复制标题
DOI：
10.1007/s11248-016-9997-6
复制
发表时间：
2017-04
影响因子：
3
通讯作者：
Gribnau J
中科院分区：
文献类型：
--
作者：
Dupont C;Loos F;Kong-A-San J;Gribnau J
尽管出现了基因组编辑工具，但ES细胞介导的转基因仍然是创造转基因动物的最可控的方式。虽然4N宿主胚胎和ES细胞的四倍体（4N）互补是保证后代完全是ES细胞衍生的唯一方法，但该技术具有挑战性，并不总是成功的，并且难以在某些实验室环境中实施。目前的研究表明，在ES细胞注射之前用FGF 4预处理宿主囊胚可以为产生显示高嵌合率的动物提供替代方法。E11胎仔和出生幼仔中的嵌合体评估显示，从着床开始，大部分产生的胚胎显示出较高的ES细胞贡献，并且发育中的幼仔不需要剖腹产分娩。
In spite of the emergence of genome editing tools, ES cell mediated transgenesis remains the most controllable way of creating genetically modified animals. Although tetraploid (4N) complementation of 4N host embryos and ES cells, is the only method guaranteeing that offspring are entirely ES cell derived, this technique is challenging, not always successful and difficult to implement in some laboratory settings. The current study shows that pretreatment of host blastocysts with FGF4 prior to ES cell injection can provide an alternative method for the generation of animals displaying high rates of chimaerism. Chimaerism assessment in E11 fetuses and born pups shows that a large percentage of resulting conceptuses show a high ES cell contribution from implantation onwards and that developing pups do not necessitate c-section for delivery.