Prevention of Chinese Sacbrood Virus Infection in Apis cerana using RNA Interference
Prevention of Chinese Sacbrood Virus Infection in Apis cerana using RNA Interference
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利用RNA干扰技术预防中华蜂病毒感染
DOI：
10.1007/s00284-010-9633-2
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发表时间：
2010-11-01
影响因子：
2.6
通讯作者：
Han, Richou
中科院分区：
文献类型：
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作者：
Liu, Xuejiao;Zhang, Yi;Han, Richou
中华蜜蜂囊状幼虫病是中华蜜蜂囊状幼虫病的病原，对中华蜜蜂构成严重威胁，易引起蜂群甚至整个蜂场的崩溃。在这里，我们报告了预防CSBV感染的喂养二龄幼虫的A。CSBV双链RNA（dsRNA）的特异性序列。通过定量实时PCR（qRT-PCR）和北方印迹分析进一步证明了通过摄取CSBV衍生的dsRNA对蜜蜂幼虫免受CSBV的保护。这一结果为保护A. CSBV感染的cerana。
Chinese sacbrood virus (CSBV) is the pathogen of Chinese sacbrood disease, which poses a serious threat to honeybee Apis cerana, and tends to cause bee colony and even the whole apiary collapse. Here we report on prevention of CSBV infection by feeding second instar larvae of A. cerana with specific sequences of CSBV double-stranded RNA (dsRNA). Protection of the bee larvae from CSBV by ingestion of CSBV-derived dsRNA was further demonstrated by quantitative real-time PCR (qRT-PCR) and northern blot analysis. The result provides a potential method to protect A. cerana from CSBV infection.