The positioning of cytoplasmic protein clusters in bacteria.
The positioning of cytoplasmic protein clusters in bacteria.
复制标题
细菌细胞质蛋白簇的定位。
DOI：
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发表时间：
2006
影响因子：
11.1
通讯作者：
J. Armitage
中科院分区：
文献类型：
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作者：
S. R. Thompson;G. Wadhams;J. Armitage
细胞分裂是一个精心策划的过程。细菌细胞具有复杂的机制来确保遗传物质被复制、校对并准确地分配到子细胞中。现在似乎某些细胞质蛋白也发生分配。此前，利用染色体荧光蛋白融合，我们证明了球形红细菌趋化蛋白的子集共定位于细菌细胞质内的离散区域。使用 TlpT 黄色荧光蛋白作为细胞质蛋白簇位置的标记，我们发现大多数细胞包含位于细胞中部的一个簇或位于细胞长度的四分之一和四分之三位置的两个簇。这些蛋白质簇的数量和定位取决于以前未被识别的细菌蛋白质定位因子 PpfA，它与细菌 I 型 DNA 分配因子具有同源性。这些数据表明，细胞分裂时某些细胞质蛋白的分配涉及一种机制，该机制类似于质粒和染色体 DNA 中所见的机制。
Cell division is a carefully orchestrated procedure. Bacterial cells have intricate mechanisms to ensure that genetic material is copied, proofread, and accurately partitioned into daughter cells. Partitioning now appears to also occur for some cytoplasmic proteins. Previously, using chromosomal fluorescent protein fusions, we demonstrated that a subset of Rhodobacter sphaeroides chemotaxis proteins colocalize to a discrete region within the bacterial cytoplasm. Using TlpT-yellow fluorescent protein as a marker for the position of the cytoplasmic protein clusters, we show most cells contain either one cluster localized at mid-cell or two clusters at the one-fourth and three-fourths positions of cell length. The number and positioning of these protein clusters depend on a previously unrecognized bacterial protein positioning factor, PpfA, which has homology to bacterial type I DNA partitioning factors. These data suggest that there is a mechanism involved in partitioning some cytoplasmic proteins upon cell division that is analogous to a mechanism seen for plasmid and chromosomal DNA.