Functionalized PCL/HA nanocomposites as microporous membranes for bone regeneration
Functionalized PCL/HA nanocomposites as microporous membranes for bone regeneration
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DOI：
10.1016/j.msec.2014.12.019
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发表时间：
2015-03-01
影响因子：
7.9
通讯作者：
Oliva, Adriana
中科院分区：
文献类型：
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作者：
Basile, Maria Assunta;d'Ayala, Giovanna Gomez;Oliva, Adriana
在本工作中，微孔膜的基础上聚（ε-己内酯）（PCL）和PCL官能化胺（PCL-DMAEA）或酸酐基团（PCL-MAGMA）实现了溶剂-非溶剂相转化，并提出用于引导组织再生（GTR）。羟基磷灰石（HA）的纳米晶须也被纳入到聚合物基体中，以实现纳米复合膜。扫描电子显微镜（SEM）显示功能化聚合物与HA的界面粘附性得到改善，并突出了孔隙率的实质性差异。提出了膜的发达的多孔结构和接枝基团的化学性质之间的关系。与原始PCL相比，官能化PCL的亲水性增加，而HA的加入仅在原始聚合物的情况下显着影响亲水特性。发现PCL-MAGMA基膜的体外降解速率显著增加，并且PCL-DMAEA膜的降解程度较低。研究了新型材料在骨修复中作为细胞生长支持的潜力，使用多能间充质基质细胞（MSC）作为模型。MSC铺板到各种膜上进行了分析的粘附，增殖和成骨能力，导致相关的化学以及多孔结构。特别是，PCL-DMAEA和相关的纳米复合膜在细胞-生物材料相互作用方面是最有前途的。(C)© 2014 Elsevier B. V.保留所有权利。
In the present work, microporous membranes based on poly(epsilon-caprolactone) (PCL) and PCL functionalized with amine (PCL-DMAEA) or anhydride groups (PCL-MAGMA) were realized by solvent-non solvent phase inversion and proposed for use in Guided Tissue Regeneration (GTR). Nanowhiskers of hydroxyapatite (HA) were also incorporated in the polymer matrix to realize nanocomposite membranes. Scanning Electron Microscopy (SEM) showed improved interfacial adhesion with HA for functionalized polymers, and highlighted substantial differences in the porosity. A relationship between the developed porous structure of the membrane and the chemical nature of grafted groups was proposed. Compared to virgin PCL, hydrophilicity increases for functionalized PCL, while the addition of HA influences significantly the hydrophilic characteristics only in the case of virgin polymer. A significant increase of in vitro degradation rate was found for PCL-MAGMA based membranes, and at lower extent of PCL-DMAEA membranes. The novel materials were investigated regarding their potential as support for cell growth in bone repair using multipotent mesenchymal stromal cells (MSC) as a model. MSC plated onto the various membranes were analyzed in terms of adhesion, proliferation and osteogenic capacity that resulted to be related to chemical as well as porous structure. In particular, PCL-DMAEA and the relative nanocomposite membranes are the most promising in terms of cell-biomaterial interactions. (C) 2014 Elsevier B.V. All rights reserved.