Serum Factors Induce C-fos Expression and Rapid Cell Proliferation in Adolescent But Not in Infant Rat Proximal Tubule Cells
Serum Factors Induce C-fos Expression and Rapid Cell Proliferation in Adolescent But Not in Infant Rat Proximal Tubule Cells
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血清因子诱导青少年大鼠近端小管细胞中的 C-fos 表达和快速细胞增殖，但在幼年大鼠中则不然
DOI：
10.1203/00006450-199103000-00008
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发表时间：
1991
影响因子：
3.6
通讯作者：
A. Aperia
中科院分区：
文献类型：
--
作者：
S. Larsson;A. Hultgårdh‐Nilsson;S. Kölare;J. Luthman;T. Sejersen;A. Aperia
摘要：研究了短期原代培养肾上皮细胞的增殖率和c-fos原癌基因的表达。实验采用分离自幼龄和青春期大鼠的肾近端小管亚汇合细胞进行。采用3h -胸腺嘧啶放射自显影法检测细胞增殖，半定量免疫荧光法检测c-fos蛋白核含量。在添加10% FCS的Dulbecco改良Eagle培养基中原代培养48 h后，婴儿和青少年肾近端小管细胞的基础增殖率相同。血清剥夺24小时对婴儿和青少年细胞的生长都有明显的抑制作用。C-fos在婴幼儿和青少年无血清细胞中表达程度相同。在婴儿和青少年细胞中，对添加血清的快速反应明显不同。在青少年细胞中，添加血清导致c-fos蛋白核表达短暂显著增加，在60雨时达到峰值。婴儿细胞中未见hi - c-fos升高。在青春期细胞中，增殖率提高了11倍，3h -胸腺嘧啶标记指数达到26.7±4.3%。在婴儿细胞中，对血清添加的增殖反应明显较低；标示指数仅为4.2±1.2%。可以排除婴儿细胞反应减弱是由于细胞死亡或代谢功能受损。结果表明，生长调节的原则在出生后发生了变化。
ABSTRACT: Kidney epithelial cells in short-term primary culture have been studied with regard to proliferative rate and expression on the c-fos protooncogene. The experiments were performed on subconfluent renal proximal tubule cells isolated from infant and adolescent rats. Proliferation was determined by 3H-thymidine autoradiography and nuclear content of c-fos protein by semiquantitative immunofluorescence. The basal proliferative rates in infant and adolescent renal proximal tubule cells were the same after 48 h of primary culture in Dulbecco's modified Eagle's medium with 10% FCS. Serum deprivation for 24 h caused a significant growth inhibition in both infant and adolescent cells. C-fos was expressed to the same extent in infant and adolescent serum-deprived cells. The rapid response to the addition of serum was markedly different in infant and adolescent cells. In adolescent cells, addition of serum led to a transient significant increase in the nuclear expression of c-fos protein, reaching a peak at 60 rain. No increase hi c-fos was seen in infant cells. In adolescent cells, the rate of proliferation increased 11-fold and 3H-thymidine labeling index reached 26.7 ± 4.3%. In infant cells, the proliferative response to serum addition was significantly lower; the labeling index reached only 4.2 ± 1.2%. It could be excluded that the attenuated response in infant cells was due to cell death or impaired metabolic function. The results imply that the principles of growth regulation change postnatally.
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DOI：
10.1152/ajpcell.1986.251.5.c815
发表时间：
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期刊：
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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