Translational Genomics in Legumes Allowed Placing In Silico 5460 Unigenes on the Pea Functional Map and Identified Candidate Genes in Pisum sativum L.
Translational Genomics in Legumes Allowed Placing In Silico 5460 Unigenes on the Pea Functional Map and Identified Candidate Genes in Pisum sativum L.
复制标题
豆类中的转化基因组学允许将硅溶液图上的硅5460个单基因放置在Pisum sativum L.中的候选基因上。
DOI：
10.1534/g3.111.000349
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发表时间：
2011-07
期刊：
影响因子：
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通讯作者：
Burstin J
中科院分区：
文献类型：
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作者：
Bordat A;Savois V;Nicolas M;Salse J;Chauveau A;Bourgeois M;Potier J;Houtin H;Rond C;Murat F;Marget P;Aubert G;Burstin J
为了鉴定与表型性状相关的基因，从高度表征的模式植物到特征不明显的作物植物的翻译基因组学提供了一个有价值的标记来源，以饱和感兴趣区域以及功能表征的候选基因。本文建立了豌豆遗传图谱的整体视图。绘制了一系列基因标记，并在白杨、白杨、大豆和白杨的伪分子上鉴定出最佳的互易同源物。根据豌豆和M. truncatula之间的合成关系，将5460个豌豆Unigenes初步置于一致性图上。开发了一种新的生物信息学工具http://www.thelegumeportal.net/pea_mtr_translational_toolkit，允许任何基因序列在豌豆共识图谱上搜索其假定位置，从而在邻近的Unigenes中搜索候选基因。例如，一个很有希望的候选基因被提出用于豌豆的高结瘤突变nod3，基于Pub1（一个参与结瘤调节的M. truncatula基因）可能的同源基因的定位。通过揭示豌豆基因组与测序基因组之间的合成关系，获得了豌豆基因组进化的更广阔视野。该研究为了解蝶蛹和菊科植物染色体结构的进化提供了新的思路。翻译基因组学方法的力量得到了强调。
To identify genes involved in phenotypic traits, translational genomics from highly characterized model plants to poorly characterized crop plants provides a valuable source of markers to saturate a zone of interest as well as functionally characterized candidate genes. In this paper, an integrated view of the pea genetic map was developed. A series of gene markers were mapped and their best reciprocal homologs were identified on M. truncatula, L. japonicus, soybean, and poplar pseudomolecules. Based on the syntenic relationships uncovered between pea and M. truncatula, 5460 pea Unigenes were tentatively placed on the consensus map. A new bioinformatics tool, http://www.thelegumeportal.net/pea_mtr_translational_toolkit, was developed that allows, for any gene sequence, to search its putative position on the pea consensus map and hence to search for candidate genes among neighboring Unigenes. As an example, a promising candidate gene for the hypernodulation mutation nod3 in pea was proposed based on the map position of the likely homolog of Pub1, a M. truncatula gene involved in nodulation regulation. A broader view of pea genome evolution was obtained by revealing syntenic relationships between pea and sequenced genomes. Blocks of synteny were identified which gave new insights into the evolution of chromosome structure in Papillionoids and Eudicots. The power of the translational genomics approach was underlined.