Conserved microRNAs miR-8-5p and miR-2a-3p modulate chitin biosynthesis in response to 20-hydroxyecdysone signaling in the brown planthopper, Nilaparvata lugens
Conserved microRNAs miR-8-5p and miR-2a-3p modulate chitin biosynthesis in response to 20-hydroxyecdysone signaling in the brown planthopper, Nilaparvata lugens
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褐飞虱 Nilaparvata lugens 中保守的 microRNA miR-8-5p 和 miR-2a-3p 响应 20-羟基蜕皮激素信号调节几丁质生物合成
DOI：
10.1016/j.ibmb.2013.06.002
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发表时间：
2013-09-01
影响因子：
3.8
通讯作者：
Zhang, Wenqing
中科院分区：
文献类型：
--
作者：
Chen, Jie;Liang, Zhikun;Zhang, Wenqing
蜕皮是昆虫重要的发育过程，通常伴随着几丁质的合成和降解。20-羟基蜕皮激素（20E）是一种昆虫激素，据报道在包括蜕皮在内的许多过程中起作用。然而，关于几丁质生物合成途径与20E信号传导之间的联系知之甚少。在这里，我们报道了保守的miR-8-5p （miR-8-5p）和miR-2a-3p及其新的靶基因对脱皮激素诱导的半翅目昆虫几丁质生物合成至关重要。通过生物信息学分析和实验验证，我们发现几丁质生物合成途径中的膜结合海藻酸酶（tre2）和磷酸乙酰氨基葡萄糖突变酶（PAGM）分别是miR-8-5p和miR-2a-3p的靶标。miR-8-5p和miR-2a-3p水平降低，而Tre-2和PAGM水平在20E的作用下上调。此外，miR-8-5p和miR-2a-3p在20E信号通路中受到早期反应基因Broad-Complex （BR-C）的转录抑制。此外，miR-8-5p和miR-2a-3p的过表达导致成活率显著降低，并伴有miR-2a-3p模拟取食引起的蜕皮障碍缺陷表型，同时褐飞虱的几丁质含量降低。因此，miR-8-5p和miR-2a-3p作为响应20E信号调节几丁质生物合成途径的分子链接。(c) 2013 Elsevier Ltd.版权所有。
Molting is an important developmental process in insects, usually along with synthesis and degradation of chitin. 20-hydroxyecdysone (20E), an insect hormone, has been reported to contribute to many processes including molting. However, little is known about the link between the chitin biosynthesis pathway and 20E signaling. Here, we report that conserved miR-8-5p (miR-8-5p) and miR-2a-3p and their new target genes are critical for ecdysone-induced chitin biosynthesis in a hemipteran insect Nilaparvata lugens. We found that membrane-bound trehalase (Tre-2) and phosphoacetylglucosamine mutase (PAGM) in the chitin biosynthesis pathway were targets of miR-8-5p and miR-2a-3p, respectively, through bioinformatic analysis and experimental verification. The levels of miR-8-5p and miR-2a-3p were reduced, whereas the levels of Tre-2 and PAGM were up-regulated in response to 20E. In addition, miR-8-5p and miR-2a-3p were transcriptionally repressed by an early-response gene, the Broad-Complex (BR-C), in the 20E signaling pathway. Moreover, the over-expression of miR-8-5p and miR-2a-3p led to a significant reduction in the survival rate along with a molting obstacles defect phenotype caused by miR-2a-3p mimics feeding, and the chitin content of N. lugens was simultaneously reduced. Thus, miR-8-5p and miR-2a-3p act as molecular link that tune the chitin biosynthesis pathway in response to 20E signaling. (c) 2013 Elsevier Ltd. All rights reserved.