Mycoplasma hyopneumoniae Surveillance in Pig Populations: Establishing Sampling Guidelines for Detection in Growing Pigs.
Mycoplasma hyopneumoniae Surveillance in Pig Populations: Establishing Sampling Guidelines for Detection in Growing Pigs.
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DOI：
10.1128/jcm.03051-20
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发表时间：
2021-04-20
影响因子：
9.4
通讯作者：
Rotolo ML
中科院分区：
文献类型：
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作者：
Clavijo MJ;Hu D;Krantz S;Cano JP;Pereira Maróstica T;Henao-Diaz A;Poeta Silva APS;Hemker D;Tapia E;Zimmerman S;Fano E;Polson D;Fitzgerald R;Tucker A;Main R;Wang C;Zimmerman JJ;Rotolo ML
猪生产系统中猪支原体感染的死前检测依赖于抗体检测，但基于DNA检测和新型诊断标本的检测的可用性，例如，气管拭子和口服液，有可能改善M。红外线监视进行了为期14周的田间研究，在此期间，在46个围栏中饲养了1，250头6周龄猪的房间中心的一个围栏中的10头猪用M.下丘脑猪生产系统中猪支原体感染的死前检测依赖于抗体检测，但基于DNA检测和新型诊断标本的检测的可用性，例如，气管拭子和口服液，有可能改善M。红外线监视进行了为期14周的田间研究，在此期间，在46个围栏中饲养了1，250头6周龄猪的房间中心的一个围栏中的10头猪用M.下丘脑此后，每隔2周从每个畜栏采集1份气管样本、4份血清样本和1份口腔液样本。对气管和口腔液样本进行M检测。猪肺炎支原体DNA和血清样本。抗猪流感抗体使用基于潜在空间分段指数生存模型的分层贝叶斯模型对检测结果进行建模，以估计畜栏内患病率、畜栏中阳性畜栏数量、样本分配、样本量和样本类型随时间变化的检测概率。分析表明，气管样本提供了最早的检测，特别是在大样本量。虽然血清样本更常收集，测试成本较低，但在大样本量下仅在暴露后30天获得高检测概率估计值。在所有情况下，30天内口腔液体的检测概率估计值明显低于气管和血清样本。最终，标本类型、样本数量和检测方法的选择将取决于检测目的和经济性，但此处提供的估计值将有助于M。针对不同情况的低气压监测和监控方案。
Antemortem detection of Mycoplasma hyopneumoniae infection in swine production systems has relied on antibody testing, but the availability of tests based on DNA detection and novel diagnostic specimens, e.g., tracheal swabs and oral fluids, has the potential to improve M. hyopneumoniae surveillance. A field study was performed over a 14-week period during which 10 pigs in one pen at the center of a room with 1,250 6-week-old pigs housed in 46 pens were intratracheally inoculated with M. hyopneumoniae. Antemortem detection of Mycoplasma hyopneumoniae infection in swine production systems has relied on antibody testing, but the availability of tests based on DNA detection and novel diagnostic specimens, e.g., tracheal swabs and oral fluids, has the potential to improve M. hyopneumoniae surveillance. A field study was performed over a 14-week period during which 10 pigs in one pen at the center of a room with 1,250 6-week-old pigs housed in 46 pens were intratracheally inoculated with M. hyopneumoniae. Thereafter, one tracheal sample, four serum samples, and one oral fluid sample were collected from every pen at 2-week intervals. Tracheal and oral fluid samples were tested for M. hyopneumoniae DNA and serum samples for M. hyopneumoniae antibody. Test results were modeled using a hierarchical Bayesian model, based on a latent spatial piecewise exponential survival model, to estimate the probability of detection by within-pen prevalence, number of positive pens in the barn, sample allocation, sample size, and sample type over time. Analysis showed that tracheal samples provided the earliest detection, especially at large sample sizes. While serum samples are more commonly collected and are less expensive to test, high probability of detection estimates were only obtained 30 days postexposure at large sample sizes. In all scenarios, probability of detection estimates for oral fluids within 30 days were significantly lower than those for tracheal and serum samples. Ultimately, the choice of specimen type, sample number, and assay will depend on testing objectives and economics, but the estimates provided here will assist in the design of M. hyopneumoniae surveillance and monitoring programs for different situations.