ENDOTHELIUM, MECHANICAL COMPLIANCE, AND CGMP CONTENT IN THE CAROTID-ARTERY FROM SPONTANEOUSLY HYPERTENSIVE RATS
ENDOTHELIUM, MECHANICAL COMPLIANCE, AND CGMP CONTENT IN THE CAROTID-ARTERY FROM SPONTANEOUSLY HYPERTENSIVE RATS
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DOI：
10.1097/00005344-199321001-00006
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发表时间：
1993-01-01
影响因子：
3
通讯作者：
LEVY, BI
中科院分区：
文献类型：
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作者：
LEGRAND, MCM;BENESSIANO, J;LEVY, BI
采用原位分离颈动脉的实验模型，对12周龄Wistar-Kyoto（WKY）大鼠和自发性高血压大鼠（SHR）的动脉壁静态力学性能进行了评价。将内皮去除对颈动脉顺应性（CC）的影响与氰化钾（KCN）完全消除血管平滑肌张力的影响进行比较。在50 - 175 mm Hg压力范围内测量的CC在接近平均动脉压的压力（工作压力）下具有最大值（WKY大鼠和SHR分别为0.22 +/- 0.07和0.13 +/- 0.03穆尔/mm Hg，p < 0.001）。在WKY大鼠和SHR中，CC的操作值分别增加了35%和45%。KCN中毒后，与对照组相比，差异有显著性（P < 0.01）。与对照值相比，内皮去除诱导CC显著增加（+37%）。在WKY大鼠和SHR中分别为p < 0.01和+25%，p < 0.01）。在内皮完整的情况下，SHR的颈动脉cGMP水平高于WKY大鼠（30.3 +/- 3.7 vs. 19.7 +/- 2.3 fmol/mg组织，p < 0.02）。去除内皮后，WKY大鼠组织cGMP减少了28%（p < 0.02），SHR减少了90%（p < 0.001）。本研究结果表明，颈动脉壁的力学性能是内皮依赖性的。在两种菌株中，内皮去除后操作压力的CC显著增加。此外，cGMP途径似乎是更多的激活在颈动脉壁从SHR比WKY大鼠。这可能被解释为对遗传异常的局部调节的内皮依赖性代偿机制不足。
An experimental model of in situ isolated carotid arteries has been used to evaluate the static mechanical properties of the arterial wall in 12-week-old Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHRs). The effects of endothelium removal on the carotid compliance (CC) were compared to the effects of total abolition of the vascular smooth muscle tone by potassium cyanide (KCN). CC measured for pressures ranging from 50 to 175 mm Hg had maximal values (0.22 +/- 0.07 and 0.13 +/- 0.03 mul/mm Hg, respectively, for WKY rats and SHRs, p < 0.001) for pressure close to the mean arterial pressure (operating pressures). Operating values of CC were increased by 35 and by 45% in WKY rats and SHRs. respectively, after KCN poisoning (p < 0.01). The endothelium removal induced a significant increase in CC compared to their control values (+37%. p < 0.01 and +25%, p < 0.01, respectively, in WKY rats and SHRs). With intact endothelium, carotid cGMP level was higher in SHRs than in WKY rats (30.3 +/- 3.7 vs. 19.7 +/- 2.3 fmol/mg tissue, p < 0.02). After endothelium removal, tissue cGMP was reduced by 28% in WKY rats (p < 0.02) and by 90% in SHRs (p < 0.001). The present results suggest that the mechanical properties of the wall of the carotid artery are endothelium-dependent. CC for operating pressure was significantly increased after endothelium removal in both strains. Furthermore, the cGMP pathway seems to be more activated in the carotid wall from SHRs than from WKY rats. This may be interpreted as an insufficient endothelium-dependent compensatory mechanism of local regulation against genetic abnormalities.