Dual modality sensor using liposome-based signal amplification technique for ultrasensitive norovirus detection
Dual modality sensor using liposome-based signal amplification technique for ultrasensitive norovirus detection
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DOI：
10.1016/j.bios.2020.112169
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发表时间：
2020-06-01
影响因子：
12.6
通讯作者：
Park, Enoch Y.
中科院分区：
文献类型：
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作者：
Ganganboina, Akhilesh Babu;Chowdhury, Ankan Dutta;Park, Enoch Y.
灵敏、准确的传染性病毒检测方法是有效疾病诊断和治疗的迫切需要。在此，基于V2 O 5纳米颗粒封装的脂质体（VONP-LP），我们展示了一种用于病毒超灵敏检测的双模态传感平台。V2 O 5纳米颗粒的电化学氧化还原特性和过氧化物酶的特性决定了其传感性能。靶特异性抗体缀合的VONP-LP和磁性纳米颗粒（MNP）通过磁性分离富集病毒，并且分离的VONP-LP结合的病毒被水解以释放包封的V2 O 5 NP。这些从捕获的脂质体释放的纳米颗粒充当过氧化物酶模拟物和电化学氧化还原指示剂，导致显著的比色和稳健的电化学双信号。利用双模态传感器具有两种定量分析形式的优势，通过电化学信号可以检测诺如病毒样颗粒（NoV-LPs），具有线性范围宽、检出限低的特点。为了验证在真实的样品中的适用性，从实际临床样品中收集的诺如病毒（NoV）以优异的准确性被有效地鉴定。该检测方法有望成为下一代病毒病早期诊断和公共卫生监测的生物检测平台。
Sensitive and accurate detection methods for infectious viruses are the pressing need for effective disease diagnosis and treatment. Herein, based on V2O5 nanoparticles-encapsulated liposomes (VONP-LPs) we demonstrate a dual-modality sensing platform for ultrasensitive detection of the virus. The sensing performance relies on intrinsic peroxidase and electrochemical redox property of V2O5 nanoparticles (V2O5 NPs). The target-specific antibody-conjugated VONP-LPs and magnetic nanoparticles (MNPs) enrich the virus by magnetic separation and the separated VONP-LPs bound viruses are hydrolyzed to release the encapsulated V2O5 NPs. These released nanoparticles from captured liposomes act as peroxidase mimics and electrochemical redox indicator resulting in noticeable colorimetric and robust electrochemical dual-signal. Utilizing the superiority of dual-modality sensor with two quantitative analysis forms, norovirus like particles (NoV-LPs) can be detected by electrochemical signals with a wide linear range and low detection limit. To verify the applicability in real samples, norovirus (NoV) collected from actual clinical samples are effectively-identified with excellent accuracy. This proposed detection method can be a promising next-generation bioassay platform for early-stage diagnosis of virus disease and surveillance for public health.