STRUCTURE AND EXPRESSION OF A HUMAN OXYTOCIN RECEPTOR
STRUCTURE AND EXPRESSION OF A HUMAN OXYTOCIN RECEPTOR
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DOI：
10.1038/356526a0
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发表时间：
1992-04-09
期刊：
影响因子：
64.8
通讯作者：
OKAYAMA, H
中科院分区：
文献类型：
--
作者：
KIMURA, T;TANIZAWA, O;OKAYAMA, H
就在临产前，子宫肌层对催产素非常敏感，因为催产素受体的数量急剧增加，子宫肌层是催产素的主要靶组织。我们在这里报告了通过表达克隆分离的人类催产素受体互补DNA的结构和表达。编码的受体是一个388个氨基酸的多肽，具有7个典型的G蛋白偶联受体的跨膜结构域。在非洲爪蟾卵母细胞中表达的催产素受体对催产素有特异性反应，并诱导向内膜电流。该受体的信使rna有两种大小，乳房中有3.6千碱基，卵巢、子宫内膜和子宫肌层中有4.4千碱基。足月时肌层的mRNA水平非常高。我们得出结论，分娩时肌层受体数量的增加，至少部分是由于mRNA的增加。
JUST before the onset of labour, uterine myometrium becomes extremely sensitive to oxytocin 1, for which it is a primary target tissue, because of a dramatic increase in the number of oxytocin receptors 2,3. We report here the structure and expression of the human oxytocin receptor complementary DNA isolated by expression cloning. The encoded receptor is a 388-amino-acid polypeptide with 7 transmembrane domains typical of G protein-coupled receptors. The oxytocin receptor, expressed in Xenopus oocytes, specifically responds to oxytocin and induces an inward membrane current. Messenger RNAs for the receptor are of two sizes, 3.6 kilobases in breast, and 4.4 kilobases in ovary, uterine endometrium and myometrium. The mRNA level in the myometrium is very high at term. We conclude that the increase in receptor number in the myometrium at labour is, at least in part, due to the increase in mRNA.