Seasonal variation of planktonic chrysophytes with special focus on Dinobryon
Seasonal variation of planktonic chrysophytes with special focus on Dinobryon
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DOI：
10.5507/fot.2014.014
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发表时间：
2014-01-01
期刊：
影响因子：
2.2
通讯作者：
Boenigk, Jens
中科院分区：
文献类型：
--
作者：
Bock, Christina;Medinger, Ralph;Boenigk, Jens
金藻的多样性往往被低估的形态调查，因为在光学显微镜下可检测的字符数量有限。解决生物多样性问题仅仅基于形态特征是进一步的问题，因为分子分析揭示了巨大的遗传多样性内形态，甚至显示在一些形态定义的物种多系。为了解决金藻的季节性变化，10个样品收集在2007年从贫营养湖Fuschlsee（萨尔茨卡默古特，奥地利）。以Dinobryon为例，比较了单细胞18 S、ITS（包括ITS 1、5.8S、部分ITS 2）和线粒体标记COI的不同基因系统发育。形态学鉴定的D. divergens和D.基于18 S rDNA序列，可以完成对巴伐利亚的分类鉴定。ITS和COI序列分析表明，该群体的遗传多样性高于18 S群体。发散细胞通过比较18 S/ITS序列数据和D. pediforme，尖叶拟步行虫D. sociale和D.巴伐利亚谱系通过将单细胞方法的结果与基于454焦磷酸测序的季节性数据相结合，我们解决了一个形态学物种在一年内在水柱中占主导地位的问题。454焦磷酸测序结果表明，D. divergens/D. bavaricum谱系显示出所有藻类/Synurophyceae中最高的丰度和强烈的季节变化，在5月和10月达到峰值。
The diversity of chrysophytes is often underestimated in morphological surveys because of the limited amount of detectable characters under the light microscope. Addressing biodiversity issues solely based on morphological characters is further problematic since molecular analyses revealed a huge genetic diversity within morphotypes, even showing polyphyly in some morphological defined species. In order to address the seasonal variations of chrysophytes, 10 samples were collected in 2007 from the oligotrophic lake Fuschlsee (Salzkammergut, Austria). By using Dinobryon as a case study, we compared different gene phylogenies of single cells of the 18S, ITS (including ITS1, 5.8S, partial ITS2) and the mitochondrial marker COI. No clear molecular phylogenetic separation between morphological identified cells of D. divergens and D. bavaricum could be accomplished based on the 18S rDNA sequences. ITS and COI sequence data revealed a higher genetic diversity then the 18S, with several independent lineages consisting of D. divergens cells. We further encountered divergent phylogenies by comparing 18S/ITS sequence data with COI data within D. pediforme, D. sociale and D. bavaricum lineages. By combining the results of the single cell approach with seasonal data based on 454 pyrosequencing, we addressed the question in how far one morphological species may dominate in the water column over the course of a single year. The results of the 454 pyrosequencing revealed that the D. divergens/D. bavaricum lineage shows the highest abundance from all Chrysophyceae/Synurophyceae and strong seasonal variations with peaks in May and October.