An Optical Trap Combined with Three-Color FRET
An Optical Trap Combined with Three-Color FRET
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DOI：
10.1021/ja408767p
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发表时间：
2013-12-11
影响因子：
15
通讯作者：
Hohng, Sungchul
中科院分区：
文献类型：
--
作者：
Lee, Sanghwa;Hohng, Sungchul
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We developed a hybrid technique combining optical tweezers and single-molecule three-color fluorescence resonance energy transfer (FRET). In demonstrative experiments, we observed the force-sensitive correlated motion of three helical arms of a Holliday junction and identified the independent unfolding/folding dynamics of two DNA hairpins of the same length. With 3 times the number of observable elements of single-molecule FRET, this new instrument will enable the measurement of the complex, multidimensional effects of mechanical forces in various biomolecular systems, such as RNA and proteins.