DISTRIBUTION OF DARPP-32 IN THE BASAL GANGLIA - AN ELECTRON-MICROSCOPIC STUDY
DISTRIBUTION OF DARPP-32 IN THE BASAL GANGLIA - AN ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1007/bf01188438
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发表时间：
1990-02-01
期刊：
影响因子：
--
通讯作者：
GREENGARD, P
中科院分区：
文献类型：
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作者：
OUIMET, CC;GREENGARD, P
DARPP-32是一种多巴胺和环腺苷酸调节的磷蛋白，已在大鼠尾壳核、苍白球和黑质中进行了光镜和电镜免疫细胞化学研究。在尾壳核中，DARPP-32存在于中型多刺型神经元中。DARPP-32的免疫反应性存在于树突棘、树突、核周细胞质、大多数但不是全部细胞核、轴突和少量轴突终末。尾壳核内的免疫反应阳性轴突终末与免疫标记的树突轴或胞体形成对称性突触。具有锯齿状核的神经元从未免疫反应。在苍白球和黑质网状部，DARPP-32存在于有髓和无髓轴突和轴突终末。标记的轴突终末在这些区域形成对称的突触接触未标记的树突轴或未标记的胞体。这些数据表明，DARPP-32是存在于纹状体神经元的中型多刺型，这些DARPP-32免疫反应神经元形成对称的突触上的靶神经元在苍白球和黑质。DARPP-32在这些纹状体神经元及其轴突终末中的存在表明DARPP-32介导纹状体中中等大小的多刺神经元对多巴胺D-1受体活化的部分反应。
DARPP-32, a dopamine and cyclic AMP-regulated phosphoprotein, has been studied by light and electron microscopical immunocytochemistry in the rat caudatoputamen, globus pallidus and substantia nigra. In the caudatoputamen, DARPP-32 was present in neurons of the medium-sized spiny type. Immunoreactivity for DARPP-32 was present in dendritic spines, dendrites, perikaryal cytoplasm, most but not all nuclei, axons and a small number of axon terminals. Immunoreactive axon terminals in the caudatoputamen formed symmetrical synapses with immunolabelled dendritic shafts or somata. Neurons having indented nuclei were never immunoreactive. In the globus pallidus and substantia nigra pars reticulata, DARPP-32 was present in myelinated and unmyelinated axons and in axon terminals. The labelled axon terminals in these reigons formed symmetrical synaptic contacts on unlabelled dendritic shafts or on unlabelled somata. These data suggest that DARPP-32 is present in striatal neurons of the medium-sized spiny type and that these DARPP-32 immunoreactive neurons form symmetrical synapses on target neurons in the globus pallidus and substantia nigra. The presence of DARPP-32 in these striatal neurons and in their axon terminals suggests that DARPP-32 mediates part of the response of medium-size spiny neurons in the striatum to dopamine D-1 receptor activation.