Quantitative trace analysis of a broad range of antiviral drugs in poultry muscle using column-switch liquid chromatography coupled to tandem mass spectrometry.
Quantitative trace analysis of a broad range of antiviral drugs in poultry muscle using column-switch liquid chromatography coupled to tandem mass spectrometry.
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DOI：
10.1007/s00216-011-5581-3
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发表时间：
2012-02
影响因子：
4.3
通讯作者：
Weigel, Stefan
中科院分区：
文献类型：
--
作者：
Berendsen, Bjorn J. A.;Wegh, Robin S.;Essers, Martien L.;Stolker, Alida A. M.;Weigel, Stefan
建立了禽肌肉中扎那米韦、利巴韦林、奥司他韦、奥司他韦羧酸盐、金刚烷胺、金刚乙胺和阿比朵尔7种抗病毒药物的液相色谱-串联质谱分析方法。用甲醇从家禽肌肉匀浆样品中提取抗病毒药物。采用串联固相萃取结合阳离子交换柱和苯基硼酸柱纯化提取物。为了防止过度的基质效应，分析物与基质成分分离使用柱切换液相色谱系统相结合的反相和Hypercarb分析柱。使用串联质谱法进行检测。根据2002/657/EC对该方法进行了充分验证，证明该方法适用于定量和确证10 μg kg−1的扎那米韦和利巴韦林，1.0 μg kg−1以下的奥司他韦、奥司他韦羧酸盐、金刚烷胺和金刚乙胺，以及1 μg kg−1以下的阿比朵尔的定性确证分析。本文的在线版本（doi：10.1007/s 00216 -011-5581-3）包含补充材料，可供授权用户使用。
A liquid chromatography–tandem mass spectrometry method for the analysis of seven antiviral drugs, zanamivir, ribavirin, oseltamivir, oseltamivir carboxylate, amantadine, rimantadine and arbidol, in poultry muscle is reported. The antiviral drugs were extracted from the homogenized poultry muscle sample using methanol. The extract was purified using tandem solid-phase extraction combining a cation exchange cartridge and a phenylboronic acid cartridge. To prevent excessive matrix effects, the analytes were separated from the matrix constituents using a column-switch liquid chromatography system combining a reversed-phase and a Hypercarb analytical column. Detection was carried out using tandem mass spectrometry. The method was fully validated according to 2002/657/EC and proved to be adequate for quantification and confirmation of zanamivir and ribavirin at 10 μg kg−1, oseltamivir, oseltamivir carboxylate, amantadine and rimantadine at levels below 1.0 μg kg−1 and for qualitative confirmatory analysis of arbidol at levels below 1 μg kg−1. The online version of this article (doi:10.1007/s00216-011-5581-3) contains supplementary material, which is available to authorized users.
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