Cell migration in cultured cerebral cortical slices.
Cell migration in cultured cerebral cortical slices.
复制标题
培养的大脑皮层切片中的细胞迁移。
DOI：
10.1006/dbio.1993.1038
复制
发表时间：
1993
影响因子：
2.7
通讯作者：
S. Mcconnell
中科院分区：
文献类型：
--
作者：
J. Roberts;N. O’Rourke;S. Mcconnell
为了研究哺乳动物大脑皮质迁移和分层的细胞机制，我们培养了发育中的端脑的活切片，并跟踪了新生成的皮质神经元的迁移。从新生雪貂皮层制成的切片培养在滚筒管中保存，并在体外存活数周。用胸腺嘧啶核苷类似物5-溴-2-脱氧尿苷(BrdU)标记出生后第0天或第1天的细胞，并在随后的培养期间跟踪它们的运动。在培养初期，标记细胞几乎仅见于脑壁增殖区、脑室和脑室下区。在培养的第一周，标记细胞戏剧性地从增殖区移动到皮质板，皮质板是新皮质神经元的最终目的地。比较培养切片和正常对照的细胞运动模式，发现标记细胞最初迁移到皮质板的过程在培养切片和正常动物中是相似的。在切片培养和体内迁移之间的主要区别是，在培养较长时间后，迁移到皮质板的细胞无法形成完整大脑中细胞特有的紧密聚集的板层。相反，标记细胞更广泛地分布在皮质板和脑壁的其他区域。因此，至少在迁移的最初阶段，培养的切片提供了一个可实验操作的系统，其中细胞迁移可以在组织正常的环境中直接观察到。
In order to investigate the cellular mechanisms of migration and lamination in the mammalian cerebral cortex, we have cultured living slices of the developing telencephalon and tracked the migration of newly generated cortical neurons. Slice cultures made from neonatal ferret cortex were maintained in roller tubes and survived well for several weeks in vitro. Cells generated on Postnatal Day (P) 0 or 1 were labeled with the thymidine analog 5-bromo-2-deoxy-uridine (BrdU), and their movements were tracked during the subsequent culture period. At the beginning of the culture period, labeled cells were found almost exclusively in the proliferative zones of the cerebral wall, the ventricular and subventricular zones. Over the first week in culture, there was a dramatic movement of labeled cells out of the proliferative zones and into the cortical plate, the final destination of neocortical neurons. Comparison of the patterns of cell movement in cultured slices with that in intact littermate controls revealed that the initial migration of labeled cells into the cortical plate is similar in cultured slices and normal animals. The primary difference between migration in slice cultures and in vivo is that after longer times in culture, cells that migrate into the cortical plate fail to form the tightly clustered laminae characteristic of cells in the intact brain. Labeled cells are instead distributed more widely throughout the cortical plate and other regions of the cerebral wall. Thus, at least during the initial period of migration, cultured slices provide an experimentally manipulable system in which cell migration can be directly observed in a histotypically normal environment.