Retention of histidine-containing peptides on a nickel affinity column.
Retention of histidine-containing peptides on a nickel affinity column.
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含组氨酸肽在镍亲和柱上的保留。
DOI：
10.1093/chromsci/45.4.207
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发表时间：
2007
影响因子：
1.3
通讯作者：
Lebl,Michal
中科院分区：
文献类型：
--
作者：
Kozlov,IgorA;Kermani,BahramG;Melnyk,PeterC;Barker,DavidL;Zhao,Chanfeng;Hachmann,JohnP;Lebl,Michal
用数百种模拟肽研究了含组氨酸肽在固定化金属亲和色谱中的保留。保留主要由组氨酸残基的数量驱动;然而，紧邻的氨基酸组成起着重要作用。具体而言，精氨酸和色氨酸含量必须考虑在内。在本研究过程中，发现了一种可与多组氨酸标签类似使用的替代标签。
The retention of histidine-containing peptides in immobilized metal-affinity chromatography is studied using several hundred modeled peptides. Retention is driven primarily by the number of histidine residues; however, the amino acid composition in the immediate vicinity plays a significant role. Specifically, the arginine and tryptophan content has to be taken into consideration. During the course of this study, an alternative tag that can be used similarly to a polyhistidine tag is discovered.