Synthesis of core-shell surface-enhanced Raman tags for bioimaging.
Synthesis of core-shell surface-enhanced Raman tags for bioimaging.
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DOI：
10.1021/ac902573p
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发表时间：
2010
影响因子：
7.4
通讯作者：
Xiangjiang Liu;M. Knauer;N. Ivleva;R. Niessner;C. Haisch
中科院分区：
文献类型：
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作者：
Xiangjiang Liu;M. Knauer;N. Ivleva;R. Niessner;C. Haisch
我们提出了一个快速和简单的核-壳表面增强拉曼散射（Sers）标签的合成过程。Sers标签是拉曼活性染料与诱导增强的金属纳米颗粒的组合。由于它们对环境条件的稳定性显著更高，大多数Sers标签被设计成核-壳几何形状。到目前为止，这种方法的主要缺点是耗时（通常是几天）和复杂的制备这些标记的核-壳颗粒。所提出的方法可以在每天的基础上在几个小时内执行。本文介绍了所制备的Sers标记物的主要特性及其在极端pH值、高离子强度和有机溶剂中的稳定性。此外，Sers标签功能化与抗沙门氏菌抗体作为一个模型，提出了一个潜在的应用核壳粒子在生物成像。
We present a rapid and straightforward procedure for the synthesis of core-shell surface-enhanced Raman scattering (SERS) tags. SERS tags are combinations of Raman active dyes with metallic nanoparticles inducing the enhancement. Because of their significantly higher stability to environmental conditions, the majority of SERS tags are designed in core-shell geometry. The main disadvantage of this approach up to now was the time-consuming (normally days) and complex preparation of these labeled core-shell particles. The presented approach can be performed within hours on a daily base. The main characteristics and the stability of the fabricated SERS tags in extreme pH values, high ion strength, or in organic solvent is demonstrated in this communication. Furthermore, the SERS tags are functionalized with anti-Salmonella antibody as a model to present a potential application of the core-shell particles in bioimaging.