Histology, ultrastructure, and differential gene expression in relation to seasonal sperm storage in the oviduct of the Chinese alligator, Alligator sinensis
Histology, ultrastructure, and differential gene expression in relation to seasonal sperm storage in the oviduct of the Chinese alligator, Alligator sinensis
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扬子鳄输卵管季节性精子储存的组织学、超微结构和差异基因表达，
DOI：
10.1071/rd21191
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发表时间：
2022-05-17
影响因子：
1.9
通讯作者：
Wu，Xiaobing
中科院分区：
文献类型：
--
作者：
Nie，Haitao;Wen，Yue;Wu，Xiaobing
虽然输卵管精子储存是内受精雌性动物生殖的重要步骤，但在鳄鱼物种中没有进行系统的研究来鉴定涉及精子储存的基因。在本研究中，与精子在输卵管中的存储相关的形态变化和来自RNA测序分析的基因表达模式之间的关系进行了研究活动期（AP），繁殖期（BP）和冬眠期（HP）。结果表明，BP期子宫漏斗部和粘膜层的纤毛细胞内可见精子，HP期子宫前部的精子贮存管（SST）内也可见精子。透射电镜分析表明，精子分泌细胞数量和活性的差异可能与精子贮存微环境的季节性变化有关。基于RNA测序，鉴定了13147个与过氧化物酶体增殖物激活受体（PPARs）和FOXO信号传导相关的DEG，包括这些，HP组中下调的ATG12和BCL 2L11因此可能构成涉及FOXO 1通路的自噬和凋亡之间的重要汇合点。参与PPARs途径的基因可能调节免疫反应，从而有助于延长扬子鳄储存精子的寿命。本研究的结果为深入了解A. sinensis。
Although oviductal sperm storage are essential steps in reproduction for female animals with internal fertilisation, no systematic study on the identification of genes involving sperm storage has been performed in crocodilian species. In the present research, the relationship between morphological variation related to sperm storage in the oviduct and gene expression patterns derived from RNA sequencing analyses between active period (AP), breeding period (BP), and hibernation period (HP) were investigated. The corresponding results indicated that sperm were observed not only in the ciliated cells within infundibulum and mucosal layer of uterus during BP, but also been detected in the spermatosperm storage tube (SST) in the anterior uterus at HP stage. The further transmission electron microscopy analysis indicated that the differences in the number and activity of the secretory cells likely to attributed to the seasonal variation of microenvironment related to the sperm storage. Based on the RNA-sequecing, 13147 DEGs related to the Peroxisome proliferator-activated receptors (PPARs) and FOXO signalling were identified, including these, the down-regulated ATG12 and BCL2L11 in the HP group may thus constitute an important point of convergence between autophagy and apoptosis involving the FOXO1 pathway. The genes involved in the PPARs pathway might modulate the immune response and thereby contribute to prolong the life span of stored spermatozoa in Alligator sinensis. The outcomes of this study provide fundamental insights into the mechanism of sperm storage in A. sinensis.