PASSIVE-IMMUNITY IN TRANSMISSIBLE GASTROENTERITIS OF SWINE - IMMUNOGLOBULIN CHARACTERISTICS OF ANTIBODIES IN MILK AFTER INOCULATING VIRUS BY DIFFERENT ROUTES
PASSIVE-IMMUNITY IN TRANSMISSIBLE GASTROENTERITIS OF SWINE - IMMUNOGLOBULIN CHARACTERISTICS OF ANTIBODIES IN MILK AFTER INOCULATING VIRUS BY DIFFERENT ROUTES
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DOI：
10.1128/iai.11.1.23-32.1975
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发表时间：
1975-01-01
影响因子：
3.1
通讯作者：
SAIF, LJ
中科院分区：
文献类型：
--
作者：
BOHL, EH;SAIF, LJ
通过不同途径对妊娠猪进行传染性胃肠炎（TGE）病毒感染，检测其血清、初乳和乳汁中抗体滴度和免疫球蛋白（IG）类别。在2 - 4日龄时，用强毒TGE病毒攻击大多数这些动物的窝，以确定被动免疫的有效性。两次经口/鼻内暴露于减毒病毒后，6只妊娠动物均未患病。牛奶中的TGE抗体主要或仅为IgG类，尽管在三只动物中检测到低水平的伊加抗体。5窝攻毒仔猪均获得一定程度的被动免疫，死亡率为25%。肌肉注射后，6怀孕的猪与强毒病毒，两种类型的临床和免疫反应，观察到，大概取决于肠道是否被感染的病毒的血源性传播。三个生病了，表现出典型的临床症状TGE，和他们的免疫反应的特点是发生在牛奶中的伊加类抗体。在仔猪攻毒后，出现了良好程度的被动免疫（0%死亡率）。相比之下，在三个怀孕的动物，没有sweat，牛奶中的抗体主要是IgG类，和穷人（69%的死亡率）的被动免疫发生。乳房内注射后的三个怀孕的猪与强毒病毒，没有观察到疾病和免疫反应的特点是发生在初乳中的高滴度的TGE抗体，主要或完全的IgG类;良好（0%死亡率）的被动免疫发生。牛奶中伊加类TGE抗体的发生与肠道感染有关，而IgG类抗体则是由肠道外抗原刺激引起的。抗原刺激的肠道提供抗体的伊加类牛奶中的作用进行了讨论。猪对TGE病毒肠道感染的被动免疫一般比IgG类的伊加抗体更完整。
Pregnant swine were exposed to transmissible gastroenteritis (TGE) virus by different routes, and their serum, colostrum, and mild were examined for titer and immunoglobulin (Ig) class of antibodies. When 2 to 4 days old, the litters of most of these animals were challenged with virulent TGE virus to determine the effectiveness of passive immunity. After two oral/intranasal exposures to attenuated virus, none of the six pregnant animals became sick. TGE antibodies in milk were primarily or solely of the IgG class, although low levels of IgA antibodies were detected in three animals. Pigs in the five challenged litters received some passive immunity, the mortality being 25%. After intramuscular injection of six pregnant swine with virulent virus, two types of clinical and immunological responses were observed, presumably dependent on whether the gut was infected by an hematogenous spread of the virus. Three became sick, showing typical clinical signs of TGE, and their immunological response was characterized by the occurrence in milk of antibodies of the IgA class. A good degree (0% mortality) of passive immunity occurred upon challenge of the suckling pigs. In contrast, in three pregnant animals that did not sicken, antibody in milk was primarily of the IgG calss, and poor (69% mortality) passive immunity occurred. After intramammary injections of three pregnant swine with virulent virus, no sickness was observed and the immunological response was characterized by the occurrence in colostrum of high titers of TGE antibodies that were primarily or solely of the IgG class; good (0% mortality) passive immunity occured. The occurrence in milk of TGE antibodies of the IgA class was associated with an intestinal infection, whereas antibodies of the IgG class resulted from a parenteral antigenic stimulation. The role of antigenic stimulation of the intestinal tract for providing antibodies in milk of the IgA class is discussed. Passive immunity against intestinal infection with TGE virus was generally more complete in pigs ingesting antibodies of the IgA than of the IgG class.