EPITHELIO-MESENCHYMAL INTERACTIONS IN PRIMORDIAL GLAND STRUCTURES WHICH BECOME RESPONSIVE TO ANDROGENIC STIMULATION
EPITHELIO-MESENCHYMAL INTERACTIONS IN PRIMORDIAL GLAND STRUCTURES WHICH BECOME RESPONSIVE TO ANDROGENIC STIMULATION
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DOI：
10.1002/ar.1091720206
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发表时间：
1972-01-01
影响因子：
--
通讯作者：
CUNHA, GR
中科院分区：
文献类型：
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作者：
CUNHA, GR
采用常规培养方法研究了雄性附性腺原基上皮和间充质之间的形态发生组织相互作用。从13 - 19日龄胚胎小鼠中切除精囊（SV）、尿生殖窦（UGS）和包皮腺（PP）雏形。胰蛋白酶分离后，将这些雏形的上皮和间充质重组为对照（同型）和实验（异型）重组体，随后在成年雄性宿主的眼前房中生长。在由附属性腺上皮（SV、UGS或PP）和泌尿生殖腺间充质（SV或UGS）组成的重组体中，重组体的组织型模式反映了上皮的来源。SV，UGS和PP上皮细胞的正常分化发生一致时，这些上皮细胞与SV或UGS间充质。另一方面，泌尿生殖腺上皮（SV或UGS）在与皮肤来源的包皮腺间充质相关时不能正常发育。相反，精囊上皮细胞保持为柱状上皮细胞排列在一个简单的管状结构，而尿生殖窦上皮细胞形成角化上皮细胞，尽管雄激素的存在。这些数据说明（1）泌尿生殖间充质的共同支持特性，（2）附属性腺上皮的稳定性，和（3）这些上皮缺乏严格的间充质要求。此外，这些数据表明，泌尿生殖系统和皮肤间充质在介导生殖依赖性发育过程的能力方面存在显著差异。
The morphogenetic tissue interactions between epithelium and mesenchyme of male accessory sexual gland primordia were investigated by standard culture methods. Seminal vesicle (SV), urogenital sinus (UGS), and preputial gland (PP) rudiments were excised from 13‐ to 19‐day old embryonic mice. After tryptic‐separation, the epithelium and mesenchyme of these rudiments were recombined into control (homotypic) and experimental (heterotypic) recombinants which were subsequently grown in the anterior chamber of the eye of adult male hosts.In recombinants composed of accessory sexual gland epithelium (SV, UGS, or PP) and urogenital gland mesenchyme (SV or UGS), the histotypic pattern of the recombinants reflected the source of the epithelium. Normal differentiation of SV, UGS, and PP epithelium occurred consistently when these epithelia were associated with SV or UGS mesenchyme. On the other hand, urogenital gland epithelium (SV or UGS) did not develop normally when associated with the integumentally‐derived preputial gland mesenchyme. Instead, seminal vesicle epithelium was maintained as a columnar epithelium arranged in a simple tubular structure, while urogenital sinus epithelium formed a keratinized epithelium despite the presence of androgens. These data illustrate (1) the supportive properties common to urogenital mesenchyme, (2) the stability of accessory sexual gland epithelium, and (3) the lack of strict mesenchymal requirements of these epithelia. Furthermore, the data suggest that urogenital and integumental mesenchyme differ significantly in their ability to mediate hormonally‐dependent developmental processes.