Influence of temperature on growth and development of dictyostelid slime moulds and its implication on the evolution of cold-tolerance
Influence of temperature on growth and development of dictyostelid slime moulds and its implication on the evolution of cold-tolerance
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DOI：
10.1101/2022.09.19.508496
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发表时间：
2022-09
期刊：
影响因子：
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通讯作者：
Hidenori Hashimura;K. Inouye
中科院分区：
文献类型：
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作者：
Hidenori Hashimura;K. Inouye
环境温度是微生物生命的主要决定因素。盘基网柄菌是土壤变形虫，能够在饥饿时进行多细胞社会行为。它们栖息在从苔原到热带的各种环境中，但它们如何适应环境温度仍然很大程度上未知。在这项研究中，研究了温度对 36 种盘基网柄菌属物种（58 株/分离株）生长和多细胞发育的影响。超过一半的物种在28°C或以上时表现出最大生长和正常发育，而有些物种可以在4°C甚至0°C下生长和发育。许多被检查的分离株来自一年中大部分时间气温远低于其首选范围的地区。热特征与系统发育之间存在显着相关性。超过 150 种已知的网柄科物种被分为几个分类组。我们的系统发育分析表明，耐冷性在主要分支中独立进化，最突出的是第 4 组（根据 Sheikh 等人的新分类为盘基网柄菌属），该组包含许多常见于亚北极地区的物种。这些结果表明，获得耐寒能力的祖先将活动范围扩大到较凉爽的地区，在那里它们可以在夏季繁殖和发育，并度过严冬。
Environmental temperature is a major determinant of microbial life. Dictyostelids are soil amoebae capable of multicellular social behaviour upon starvation. They inhabit in a variety of environments from the tundra to the tropics, but how they have adapted to environmental temperature remains largely unknown. In this study, the effects of temperature on the growth and multicellular development of 36 dictyostelid species (58 strains/isolates) were examined. More than half of the species showed maximal growth and normal development at 28°C or above, whereas some could grow and develop at 4°C, or even at 0°C. Many of the isolates examined were from areas with temperatures far lower than their preferred range over a large part of the year. There was a significant correlation between thermal characteristics and phylogeny. Over 150 known dictyostelid species are divided into several taxonomic groups. Our phylogenetic analysis indicated that cold-tolerance evolved independently in major clades, most prominently in group 4 (genus Dictyostelium according to the new classification by Sheikh et al.), which contains many species that are often found in subarctic regions. These results suggest that ancestors that have acquired cold-tolerance expanded their ranges into cooler areas where they could proliferate and develop during summer and survive the severe winter.