Cytonuclear genic incompatibilities cause increased mortality in male F2 hybrids of Nasonia giraulti and N-vitripennis
Cytonuclear genic incompatibilities cause increased mortality in male F2 hybrids of Nasonia giraulti and N-vitripennis
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DOI：
10.1534/genetics.107.080523
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发表时间：
2008-01-01
期刊：
影响因子：
3.3
通讯作者：
Gadau, Juergen
中科院分区：
文献类型：
--
作者：
Niehuis, Oliver;Judson, Andrea K.;Gadau, Juergen
单倍体黄蜂属（Nasonia）是一种很有前途的模型，用于研究由于存在干涉种和单倍体雄性而导致的基因不亲和性进化。后者可以显著减少检测和绘制隐性功能失调基因相互作用所需的样本量。利用这些特征，研究了黄颡鱼（Nasonia giraulti）和黄颡鱼（N. vitripennis） F-2雄性杂种内在杂种不育的遗传特性。通过对225个杂交胚胎的标记分离分析，我们推断出了一个包含38个框架标记的连锁图谱。对这些标记进行了标记传递比失真（MTRD）和染色体间连锁不平衡的检测。我们发现了四个传输比扭曲位点（TRDL）的证据。3个TRDL显示，在具有玻纤蕨细胞质的杂交种中，giraulti等位基因缺失。一个单独的TRDL显示了X玻璃鸟等位基因的缺陷与X giraulti细胞质杂交。我们将观察到的成年杂种的MTRD归因于细胞核基因不相容，这导致了杂种胚胎在发育过程中死亡率的差异，因为杂种胚胎没有表现出MTRD。在该杂交中，鉴定出的F2杂种与玻璃翅蕨细胞质的细胞核基因不亲和性是该杂交内在无活力的主要原因。然而，具有吉拉氏白僵菌细胞质的F2杂交种的高死亡率不能仅用鉴定出的单一TRDL来解释。
The haplodiploid wasp genus Nasonia is a promising model for studying the evolution of genic incompatibilities due to the existence of interfertile species and haploid males. The latter allows for significantly reducing the sample size required to detect and map recessive dysfunctional genic interactions. We exploited these features to study the genetics of intrinsic hybrid inviability in male F-2 hybrids of Nasonia giraulti and N. vitripennis. Analyzing marker segregation in 225 hybrid embryos, we inferred a linkage map with 38 framework markers. The markers were tested for marker transmission ratio distortion (MTRD) and interchromosomal linkage disequilibrium in populations of embryonic and adult hybrids. We found evidence for four transmission ratio distorting loci (TRDL). Three TRDL showed a deficit of the N. giraulti allele in hybrids with N vitripennis cytoplasm. A separate TRDL exhibited a deficiency of the X vitripennis allele in hybrids with X giraulti cytoplasm. We ascribe the observed MTRD in adult hybrids to cytonuclear genic incompatibilities causing differential mortality during development since hybrid embryos did not show MTRD. The identified cytonuclear genic incompatibilities in F2 hybrids with N. vitripennis cytoplasm account for most of the intrinsic hybrid inviability in this cross. The high mortality rate in F2 hybrids with N. giraulti cytoplasm cannot be explained by the single identified TRDL alone, however.