Review of methods to assign the nuclear magnetic resonance peaks of reductively methylated proteins.
Review of methods to assign the nuclear magnetic resonance peaks of reductively methylated proteins.
复制标题
回顾还原甲基化蛋白质的核磁共振峰的分配方法。
DOI：
10.1016/j.ab.2014.08.024
复制
发表时间：
2014
影响因子：
2.9
通讯作者：
Macnaughtan,MeganA
中科院分区：
文献类型：
--
作者：
Roberson,KevinJ;Macnaughtan,MeganA
赖氨酸侧链胺的还原甲基化已成为高分辨率结构生物学研究的成功工具。这种方法的应用在蛋白质化学修饰方面不断取得进展，首先作为帮助蛋白质结晶的工具，后来作为蛋白质核磁共振（NMR）光谱的探针。作为核磁共振研究的一种同位素标记策略，还原甲基化有助于蛋白质-蛋白质相互作用和整体构象变化的研究。尽管使用这种标记策略进行更详细的结构研究是可能的，但将核磁共振峰分配给相应的还原甲基化胺的障碍阻碍了它的使用。在这篇综述中，我们讨论和比较了用于分配还原甲基化蛋白胺的核磁共振峰的策略。
Reductive methylation of lysyl side-chain amines has been a successful tool in the advancement of high-resolution structural biology. The utility of this method has continuously gained ground as a protein chemical modification, first as a tool to aid protein crystallization and later as a probe in protein nuclear magnetic resonance (NMR) spectroscopy. As an isotope-labeling strategy for NMR studies, reductive methylation has contributed to the study of protein–protein interactions and global conformational changes. Although more detailed structural studies using this labeling strategy are possible, the hurdle of assigning the NMR peaks to the corresponding reductively methylated amine hinders its use. In this review, we discuss and compare strategies used to assign the NMR peaks of reductively methylated protein amines.