Rat small-intestinal beta-galactosidases. Separation by ion-exchange chromatography and gel filtration.
Rat small-intestinal beta-galactosidases. Separation by ion-exchange chromatography and gel filtration.
复制标题
大鼠小肠β-半乳糖苷酶。
DOI：
10.1042/bj1060841
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
A. Dahlqvist
中科院分区：
文献类型：
--
作者：
N. Asp;A. Dahlqvist
1. The chromatography of rat small-intestinal beta-galactosidase activities on gel-filtration and ion-exchange columns has been studied. Five different substrates were used to measure beta-galactosidase activity (lactose, phenyl beta-galactoside, o-nitrophenyl beta-galactoside, p-nitrophenyl beta-galactoside and 6-bromo-2-naphthyl beta-galactoside) and the activity was measured at one acid and one more neutral pH value. 2. By gel filtration one acid beta-galactosidase, hydrolysing lactose and the hetero-beta-galactosides at about the same rate, and one more neutral beta-galactosidase, hydrolysing lactose much more rapidly than the hetero-beta-galactosides, were separated. 3. By ion-exchange chromatography the acid enzyme was fractionated into two components. These may be individual enzymes or different forms of the same enzyme.
