Blood-borne stem cells differentiate into vascular and cardiac lineages during normal development.
Blood-borne stem cells differentiate into vascular and cardiac lineages during normal development.
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血源性干细胞在正常发育过程中分化成血管和心脏谱系。
DOI：
10.1089/scd.2006.15.17
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Sedmera,David
中科院分区：
文献类型：
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作者：
Zhang,Ning;Mustin,Deanna;Reardon,Wade;Almeida,AngelaDe;Mozdziak,Paul;Mrug,Michal;Eisenberg,LeonardM;Sedmera,David
近年来的研究表明，造血干细胞（hematopoietic stem cells，HSCs）具有分化为多种非血细胞谱系的潜能，可促进多种组织和器官的细胞再生。迄今为止，大多数关于HSC潜力的研究都检查了成人，重点是它们在病理条件下修复组织的潜力（例如，缺血性损伤、器官衰竭）。相对较少的是已知的造血干细胞在正常组织稳态的生理作用，在成人，甚至更少的贡献，在产前发育的器官发生。本研究报告的贡献，血液来源的细胞的各种器官系统的发展胚胎使用鹌鹑鸡共生模型。在这些条件下，发育中的循环系统在ED 6-ED 8之间融合，导致循环细胞的自由交换。鹌鹑来源的细胞，确定鹌鹑特异性抗体在ED 15，被发现在许多器官的联体鸡胚。循环细胞有助于血管系统的发育，在那里它们分化成内皮、平滑肌和外膜组织。在心脏中，循环细胞分化为心肌细胞，证明使用双免疫标记QCPN和肌节肌动蛋白或肌球蛋白。心肌内注射鹌鹑骨髓细胞证实了这些结果，这些细胞被发现表达肌细胞、冠状动脉平滑肌和心外膜的标志物。利用lacZ转基因鸡胚作为第二个阳性细胞标记的实验表明，鸡和鹌鹑细胞之间的融合是罕见的事件。这些结果表明，在发育过程中，多能细胞存在于胚胎循环中，并进入不同的器官，在那里它们经历组织特异性分化。此外，造血干细胞有助于各种器官发育的证明，使造血干细胞可用于治疗成人患病或受损组织的说法更加可信。
Recent investigations have indicated that hematopoietic stem cells (HSCs) have the potential to differentiate into multiple non-blood cell lineages and contribute to the cellular regeneration of various tissues and multiple organs. Most studies to date on HSC potential have examined the adult, focusing on their potential to repair tissue under pathological conditions (e.g., ischemic injury, organ failure). Comparatively little is known about the physiological role of HSCs in normal tissue homeostasis in the adult, and even less of their contribution to organogenesis during prenatal development. This study reports the contribution of blood-borne cells to various organ systems of the developing embryo using a quail–chick parabiosis model. Under these conditions, the developing circulatory systems fuse between ED6–ED8, resulting in free exchange of circulating cells. Cells of quail origin, identified by quail-specific antibodies at ED15, were found in numerous organs of the parabiotic chick embryo. Circulating cells contributed to developing vasculature, where they differentiated into endothelial, smooth muscle, and adventitial tissues. In the heart, differentiation of circulating cells into cardiomyocytes was demonstrated using double immunolabeling for QCPN and sarcomeric actin or myosin. These results were confirmed by intramyocardial injection of quail bone marrow cells that were found to express markers of myocytes, coronary smooth muscle, and epicardium. Experiments using lacZ-transgenic chick embryos for a second positive cellular marker showed that fusion between chick and quail cells was a rare event. These results suggest that during development, multipotent cells are present in the embryonic circulation and home into different organs where they undergo tissue-specific differentiation. Moreover, the demonstration that blood-borne cells contribute to the development of various organs lends credence to claims that hematopoietic stem cells have utility for treating diseased or damaged tissues in the adult.