RESIDUAL ANTIGENICITY OF HYPOALLERGENIC INFANT FORMULAS AND THE OCCURRENCE OF MILK-SPECIFIC IGE ANTIBODIES IN PATIENTS WITH CLINICAL ALLERGY
RESIDUAL ANTIGENICITY OF HYPOALLERGENIC INFANT FORMULAS AND THE OCCURRENCE OF MILK-SPECIFIC IGE ANTIBODIES IN PATIENTS WITH CLINICAL ALLERGY
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DOI：
10.1016/s0091-6749(95)70056-0
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发表时间：
1995-09-01
影响因子：
14.2
通讯作者：
SCHMIDT, DG
中科院分区：
文献类型：
--
作者：
VANBERESTEIJN, ECH;MEIJER, RJGM;SCHMIDT, DG
背景：牛奶蛋白水解物经常用于牛奶过敏儿童的牛奶替代品中。目的：检测牛奶过敏患者血清中乳蛋白水解物和8种婴幼儿配方奶粉对不合格乳蛋白水解物的免疫学反应。方法：用间接和竞争性ELISA法检测患者血清中的抗体水平和乳蛋白特异性残留抗原性。结果：牛奶过敏患者血清中存在针对牛乳几种蛋白组分的IgE和IGE抗体，这些抗体的浓度存在较大的个体内和个体间差异。总体而言，低变应原产品中单个乳蛋白的IgE和Ig G残存抗原性低于完整乳蛋白，但在所有产品中仍可检测到免疫反应表位。结论：牛乳过敏患者的个体致敏模式和乳蛋白特异性残留抗原性可作为低变应原产品不良反应的实验室预测指标。他们的测定可能是儿科医生选择适合个别患者的配方的有用的临床前筛查试验。
Background: Milk protein hydrolysates are frequently used in milk substitutes for children with cow's milk allergy. However, cases of hypersensitivity to commercially available hypoallergenic infant formulas based on milk protein hydrolysates have been reported.Objective: Our purpose was to determine the immunologic response of milk protein-specific IgE and IgG in the serum of patients allergic to cow's milk against foul commercially available hypoallergenic milk protein hydrolysates and eight infant formulas.Methods: Antibody levels in patients' serum and milk protein-specific residual antigenicity of the hypoallergenic products were determined by indirect and competitive ELISA.Results: Patients allergic to cow's milk had IgE and Ige antibodies to several protein fractions of cow's milk; intraindividual and interindividual variation in the concentrations of these antibodies was considerable. In general, IgE and IgG residual antigenicity of individual milk proteins in the hypoallergenic products was lower compared with that of the intact milk protein bur immunoreactive epitopes could still be detected in all products. Their number varied considerably among the individual milk proteins and also differed among products.Conclusions: The individual sensitization pattern of the patient allergic to cow's milk and the milk protein-specific residual antigenicity might be considered as possible laboratory predictors of adverse reactions to hypoallergenic products. Their determination could be a useful preclinical screening test for pediatricians to select a formula adapted to the individual patient.