Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells
Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells
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DOI：
10.1242/dev.01173
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发表时间：
2004-07-01
期刊：
影响因子：
4.6
通讯作者：
Ogawa, M
中科院分区：
文献类型：
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作者：
Miyata, T;Kawaguchi, A;Ogawa, M
成熟的新皮质层都来自皮质板（CP），这是一个位于端脑背侧的短暂区域，年轻的神经元不断地被输送到该区域。为了了解触发CP出现的细胞遗传学和组织遗传学事件，我们使用了切片培养技术。大多数分裂在心室表面产生成对的循环女儿（P/P分裂）和大多数的P/P分裂是不对称的子细胞的行为，他们经常发送一个子细胞的非表面（NS）的位置，室管膜下区（SVZ），在一个单一的细胞周期长度，而保持其他有丝分裂的女儿分裂在表面。NS分裂细胞大多数是Hu（+），它们的子细胞也是Hu+，表明它们致力于神经元谱系，并在比心室表面更接近其命运的位置供应早期神经元。通过细胞的心室突的塌陷，随后通过其NS分裂来实现将循环子细胞释放到SVZ。神经生成素2（Neurogenin 2，Ngn 2）在G1期的特定循环群体中被免疫组化检测到，并在G2-M期进一步被限制在SVZ定向群体中。其逆转录病毒的引入将表面分裂转化为NS分裂。因此，不对称的P/P分裂可能有助于通过细胞周期依赖性和谱系限制性Ngn 2表达的CP启动所需的有效神经元/祖细胞分离。
Mature neocortical layers all derive from the cortical plate (CP), a transient zone in the dorsal telencephalon into which young neurons are continuously delivered. To understand cytogenetic and histogenetic events that trigger the emergence of the CP, we have used a slice culture technique. Most divisions at the ventricular surface generated paired cycling daughters (P/P divisions) and the majority of the P/P divisions were asymmetric in daughter cell behavior; they frequently sent one daughter cell to a non-surface (NS) position, the subventricular zone (SVZ), within a single cell-cycle length while keeping the other mitotic daughter for division at the surface. The NS-dividing cells were mostly Hu(+) and their daughters were also Hu+, suggesting their commitment to the neuronal lineage and supply of early neurons at a position much closer to their destiny than from the ventricular surface. The release of a cycling daughter cell to SVZ was achieved by collapse of the ventricular process of the cell, followed by its NS division. Neurogenin2 (Ngn2) was immunohistochemically detected in a certain cycling population during G1 phase and was further restricted during G2-M phases to the SVZ-directed population. Its retroviral introduction converted surface divisions to NS divisions. The asymmetric P/P division may therefore contribute to efficient neuron/progenitor segregation required for CP initiation through cell cycle-dependent and lineage-restricted expression of Ngn2.