Midkine Expression in Human Periapical Granulomas
Midkine Expression in Human Periapical Granulomas
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DOI：
10.1016/j.joen.2011.03.009
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发表时间：
2011-06-01
影响因子：
4.2
通讯作者：
Komiyama, Kazuo
中科院分区：
文献类型：
--
作者：
Hatori, Keisuke;Takeichi, Osamu;Komiyama, Kazuo
简介：中期因子（MK）是一种肝素结合生长因子，在多种人类肿瘤中表达增加，使其成为一种有前途的肿瘤标志物和肿瘤治疗靶点。MK也与慢性或自身免疫性疾病的发展和病因的调节有关;然而，MK在根尖牙周炎中的参与从未被检查过。本研究比较了根尖周肉芽肿和健康牙龈组织中MK表达细胞和MK信使RNA表达的定位。研究方法：手术切除慢性根尖周炎患者的根尖周病变，用苏木精-伊红染色连续组织切片。病理诊断为根尖肉芽肿的病变用人MK单克隆抗体进行免疫组化检查。MK信使RNA表达也采用实时聚合酶链反应分析。以相同的方式分析健康牙龈组织。结果如下：MK表达的炎症细胞，如巨噬细胞，淋巴细胞和中性粒细胞，以及在根尖肉芽肿的内皮细胞，但不是在健康的牙龈组织。在血管周围可见MK表达的炎性细胞，血管周围含有MK表达的内皮细胞，表明在炎性细胞浸润过程中MK在这些细胞之间相互作用。MK信使RNA表达的定量分析显示，根尖周肉芽肿组织中MK的表达明显高于健康牙龈组织。结论：MK可能参与根尖肉芽肿的发病过程。（J Endod 2011;37：781-785）
Introduction: The expression of midkine (MK), a heparin-binding growth factor, is increased in various human tumors, making it a promising tumor marker and target for tumor therapy. MK is also related to the regulation of the development and etiology of chronic or autoimmune diseases; however, the involvement of MK in apical periodontitis has never been examined. This study compared the localization of MK-expressing cells and MK messenger RNA expression in periapical granulomas with healthy gingival tissues. Methods: Periapical lesions were removed surgically from chronic apical periodontitis patients, and serial tissue sections were stained with hematoxylin-eosin. The lesions diagnosed as periapical granulomas pathologically were examined by immunohistochemistry using human MK monoclonal antibodies. MK messenger RNA expression was also detected using real-time polymerase chain reaction analysis. Healthy gingival tissues were analyzed in the same manner. Results: MK was expressed by inflammatory cells, such as macrophages, lymphocytes, and neutrophils, as well as by endothelial cells in periapical granulomas but not in healthy gingival tissues. The MK-expressing inflammatory cells were seen adjacent to blood vessels, which contained MK-expressing endothelial cells, suggesting the interaction of MK among these cells during the process of inflammatory cell infiltration. Quantitative analysis of MK messenger RNA expression revealed that periapical granulomas expressed significantly more MK than healthy gingival tissues. Conclusions: These findings suggest that MK is involved in the pathogenesis of periapical granulomas. (J Endod 2011;37:781-785)