Mutation of the cohesin related gene PDS5 causes cell death with predominant apoptotic features in Saccharomyces cerevisiae during early meiosis
Mutation of the cohesin related gene PDS5 causes cell death with predominant apoptotic features in Saccharomyces cerevisiae during early meiosis
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DOI：
10.1016/j.mrfmmm.2004.11.014
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发表时间：
2005-03-01
影响因子：
2.3
通讯作者：
Zhang, ZJ
中科院分区：
文献类型：
--
作者：
Ren, Q;Yang, H;Zhang, ZJ
Pds 5 p是一种粘着蛋白相关蛋白。在有丝分裂和减数分裂中，它是维持姐妹染色单体凝聚所必需的。在这里，我们报告说，胶5 -1导致细胞死亡的酵母酿酒酵母在减数分裂早期。致细胞死亡具有凋亡和坏死的特征，包括磷脂酰丝氨酸在细胞质膜外化、DNA断裂累积、染色质浓缩和断裂、细胞核断裂、膜变性和细胞体积增大。我们的研究结果还表明，（1）DNA修复缺陷;（2）活性氧的产生，在<$5 -1突变体中参与了<$5 -1诱导的细胞死亡。(C)2004 Elsevier B. V.保留所有权利。
Pds5p is a cohesin related protein. It is required for maintenance of sister chromatid cohesion in mitosis and meiosis. Here we report that pds5-1 causes cell death in yeast Saccharomyces cerevisiae during early meiosis. The pds5-1 caused cell death possesses characteristics of apoptosis and necrosis, including externalization of phosphatidylserine at cytoplasmic membrane, accumulation of DNA breaks, chromatin condensation and fragmentation, nuclei fragmentation, membrane degeneration and cell size enlargement. Our results also suggest that (1) The defect of DNA repair; (2) The production of reactive oxygen species, in pds5-1 mutant are involved in pds5-1 induced cell death. (C) 2004 Elsevier B.V. All rights reserved.