SUMO‐1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation
SUMO‐1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation
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DOI：
10.1002/mrd.22339
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发表时间：
2014-08
影响因子：
2.5
通讯作者：
Yi‐Feng Yuan;R. Zhai;Xiao-ming Liu;Hamid Ali Khan;Yanhong Zhen;L. Huo
中科院分区：
文献类型：
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作者：
Yi‐Feng Yuan;R. Zhai;Xiao-ming Liu;Hamid Ali Khan;Yanhong Zhen;L. Huo
许多靶蛋白的小泛素相关修饰因子- 1 （SUMO - 1）依赖性修饰参与了一系列细胞内过程。先前的研究报道了SUMO - 1在卵母细胞减数分裂期间的定位，以及Sentrin/SUMO特异性蛋白酶2（一种去SUMO化蛋白酶）的过表达，改变了SUMO修饰的蛋白，并导致中期II纺锤体组织的缺陷。在这项研究中，我们通过特定抗体抑制SUMO‐1或UBC9，或通过特异性siRNA显微注射去除它们，详细介绍了SUMO‐1介导的SUMO‐1介导的SUMO‐1酰化的后果。在生发囊泡（GV）期卵母细胞中抑制或耗尽SUMO‐1或UBC9可降低生发囊泡破裂和第一极体（PB1）挤压的率；导致纺锤体组织缺陷和染色体错位；导致成熟卵母细胞的非整倍体。阶段特异性抗体注射表明，SUMO‐1在PB1挤压的I期后期之前起作用。进一步的实验表明，SUMO‐1抑制后γ -微管蛋白的定位紊乱，SUMO‐1的缺失破坏了中期的着丝点微管附着。此外，SUMO‐1抑制导致染色体凝聚度降低，REC8和securin的定位改变，并减少了着丝点BUBR1的积累。另一方面，在GV期卵母细胞中过表达SUMO‐1对卵母细胞成熟没有显著影响。综上所述，我们的研究结果表明SUMO‐1在卵母细胞减数分裂成熟过程中起着至关重要的作用，特别是涉及纺锤体组装和染色体行为，通过调节着丝粒-微管附着和γ -微管蛋白、BUBR1、REC8和securin的定位。摩尔。天线转换开关。开发学报，2014:712-724。©2014 Wiley期刊公司
Small ubiquitin‐related modifier‐1 (SUMO‐1)‐dependent modifications of many target proteins are involved in a range of intracellular processes. Previous studies reported the localization of SUMO‐1 during oocyte meiosis, and that overexpression of Sentrin/SUMO‐specific protease 2 (SENP2), a de‐SUMOylation protease, altered SUMO‐modified proteins, and caused defects in metaphase‐II spindle organization. In this study, we detailed the consequences of SUMO‐1‐mediated SUMOylation by either inhibition of SUMO‐1 or UBC9 with a specific antibody or their depletion by specific siRNA microinjection. Inhibition or depletion of SUMO‐1 or UBC9 in germinal vesicle (GV)‐stage oocytes decreased the rates of germinal vesicle breakdown and first polar body (PB1) extrusion; caused defective spindle organization and misaligned chromosomes; and led to aneuploidy in matured oocytes. Stage‐specific antibody injections suggested that SUMO‐1 functions before anaphase I during PB1 extrusion. Further experiments indicated that the localization of γ‐tubulin was disordered after SUMO‐1 inhibition, and that SUMO‐1 depletion disrupted kinetochore‐microtubule attachment at metaphase I. Moreover, SUMO‐1 inhibition resulted in less‐condensed chromosomes, altered localization of REC8 and securin, and reduced BUBR1 accumulation at the centromere. On the other hand, overexpression of SUMO‐1 in GV‐stage oocytes had no significant effect on oocyte maturation. In conclusion, our results implied that SUMO‐1 plays crucial roles during oocyte meiotic maturation, specifically involving spindle assembly and chromosome behavior, by regulating kinetochore‐microtubule attachment and the localization of γ‐tubulin, BUBR1, REC8, and securin. Mol. Reprod. Dev. 81: 712–724, 2014. © 2014 Wiley Periodicals, Inc.