Isolation and identification of stem cells in different subtype of cartilage tissue
Isolation and identification of stem cells in different subtype of cartilage tissue
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不同亚型软骨组织干细胞的分离与鉴定
DOI：
10.1517/14712598.2015.989207
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发表时间：
2015-05-01
影响因子：
4.6
通讯作者：
Liu, Kai
中科院分区：
文献类型：
--
作者：
Xue, Ke;Xia, Wanyao;Liu, Kai
背景：软骨组织工程为软骨缺损的修复提供了一种有前景的疗法，接种细胞在软骨再生中起着至关重要的作用。据报道，软骨细胞和骨髓间充质干细胞（BMSCs）是理想的接种细胞，但这两种细胞构建的组织工程软骨的“去分化”和“不稳定表型”阻碍了它们的临床应用。最近，有报道称软骨组织中存在干细胞群，这可能是软骨组织工程中更优越的细胞来源。
方法：在本研究中，我们通过对纤连蛋白的差异黏附试验从不同亚型的软骨组织中分离出一个细胞群。
结果：流式细胞术分析表明，这些细胞系表达间充质干细胞阳性表面标志物，如CD29和CD90。同时，这些细胞具有高度增殖能力和多能性。逆转录 - 聚合酶链反应检测显示，该细胞群在不同的诱导条件下可表达成骨和成脂分化。更有趣的是，在地塞米松和胰岛素样生长因子1存在的情况下，单层细胞可发生软骨分化。此外，软骨来源干细胞（CSCs）中软骨形成基因的表达高于BMSCs。
结论：由于其干性和软骨形成特性，CSC可能成为软骨组织工程中理想的接种细胞。
Background: Cartilage tissue engineering provided a promising therapy for the repair of cartilage defects, and seeding cells play a vital role in cartilage regeneration. Chondrocytes and bone marrow-derived mesenchymal stem cells (BMSCs) were reported to be the ideal seeding cells, but 'dedifferentiation' and 'unstable phenotype' of tissue-engineered cartilage constructed by the two cell type hamper their clinical application. Recently, cartilage tissue was reported to possess a stem cell population, which may be a more superior cell source in cartilage tissue engineering.Methods: In current study, we isolated a cell population from different subtype of cartilage tissue via a differential adhesion assay to fibronectin.Results: Flow cytometry analysis demonstrates the cell lines expressed mesenchyme stem cell positive surface marker such as CD29 and CD90. Meanwhile, the cells are highly proliferative and multipotent. Reverse transcription-PCR detection showed the cell population expressed osteogenic and adipogenic differentiation under different induction conditions. More interesting, monolayer cells underwent chondrogenic differentiation in the presence of dexamethasone and insulin-like growth factor 1. In addition, the expression of chondrogenic genes in cartilage-derived stem cells (CSCs) was higher than those in BMSCs.Conclusion: CSC may become an ideal seeding cell in cartilage tissue engineering, owing to its stemness and chondrogenic characteristics.