MONOCLONAL-ANTIBODIES AGAINST HUMAN MILK-FAT GLOBULE MEMBRANES DETECTING DIFFERENTIATION ANTIGENS OF THE MAMMARY-GLAND AND ITS TUMORS
MONOCLONAL-ANTIBODIES AGAINST HUMAN MILK-FAT GLOBULE MEMBRANES DETECTING DIFFERENTIATION ANTIGENS OF THE MAMMARY-GLAND AND ITS TUMORS
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DOI：
10.1002/ijc.2910340210
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发表时间：
1984-01-01
影响因子：
6.4
通讯作者：
VANDERVALK, M
中科院分区：
文献类型：
--
作者：
HILKENS, J;BUIJS, F;VANDERVALK, M
用鼠抗人乳脂球膜（HMFGM）单克隆抗体制备乳腺肿瘤诊断试剂。选择一组17种抗HMFGM抗体[40 A5、67 B7、115 A7、40 C2、67 H11、115 E3、67 D11、115 H10、115 C2、115 E6、115 G3、67 D2、67 D9、67 F12、115 D8、115 G2和115 F5]用于进一步研究。抗体阻断研究表明，使用这些抗体，可以在6种不同的分子MAM-I至MAM-6上区分至少9种不同的非重叠表位。EM研究的细胞定位的抗原检测这些抗体中的一些显示，它们主要存在于细胞膜上，在微绒毛，内衬细胞间和胞浆内腔。在正常和肿瘤组织上以及在体外细胞系上研究抗体的反应性。所有抗体均与静息乳腺反应，而8种抗体也与乳腺肿瘤结合。没有一种抗体仅对乳腺或其肿瘤具有特异性，但大多数抗体也与其他上皮细胞，特别是分泌组织的上皮细胞反应。当在多种细胞系上进行测试时，可以证明反映组织分布的分布。其中一种抗体与几乎所有的癌及其转移灶反应，而与淋巴瘤、肉瘤、神经母细胞瘤、黑色素瘤或神经系统肿瘤不反应。单独测试的抗体的特异性不足以进一步鉴别诊断癌症，但是当这些抗体中的一些用于面板中时，它们有助于诊断的重要改进。
Mouse monoclonal antibodies were against human milk-fat globule membranes (HMFGM) to obtain reagents for mammary tumor diagnosis. A panel of 17 anti-HMFGM antibodies [40A5, 67B7, 115A7, 40C2, 67H11, 115E3, 67D11, 115H10, 115C2, 115E6, 115G3, 67D2, 67D9, 67F12, 115D8, 115G2 and 115F5] was selected for further investigation. Antibody-blocking studies indicated that with these antibodies at least 9 different non-overlapping epitopes could be distinguished on 6 different molecules, MAM-I to MAM-6. EM studies of the cellular localization of the antigens detected by some of these antibodies revealed that they were present on the cell membrane mainly, on the microvilli, lining intercellular and intracytoplasmic lumina. The reactivity of the antibodies was studied on normal and tumor tissues and on in vitro cell lines. All antibodies reacted with the resting mammary gland while 8 antibodies also bound to breast tumors. None of the antibodies was specific for the mammary gland or its tumors only, but most antibodies also reacted with other epithelial cells, especially of secretory tissues. When tested on a variety of cell lines a distribution reflecting the tissue distribution could be demonstrated. One of the antibodies reacted with nearly all carcinomas and their metastases and did not react with lymphomas, sarcomas, neuroblastomas, melanomas or nervous system tumors. The specificity of the antibodies, tested individually, was not sufficient for further differential diagnosis of the carcinomas, but when some of these antibodies were used in a panel they contribute to an important improvement of the diagnosis.