Evaluation of extracellular lipid peroxidation in brain cortex of anaesthetized rats by microdialysis perfusion and high-performance liquid chromatography with fluorimetric detection
Evaluation of extracellular lipid peroxidation in brain cortex of anaesthetized rats by microdialysis perfusion and high-performance liquid chromatography with fluorimetric detection
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DOI：
10.1016/s0378-4347(97)00033-9
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发表时间：
1997-06-06
影响因子：
3
通讯作者：
Kuo, JS
中科院分区：
文献类型：
--
作者：
Yang, CS;Tsai, PJ;Kuo, JS
建立了一种在体评价麻醉大鼠脑皮质细胞外间隙脂质过氧化反应的方法。该方法包括微透析灌流和高效液相色谱法。与硫代巴比妥酸（TBA）反应，然后通过配备有荧光检测器的HPLC系统进行分析（激发和发射波长分别为515和550 nm），细胞外间隙中的脂质过氧化反应以脂质过氧化终产物丙二醛的浓度进行评价，所述脂质过氧化终产物与TBA反应形成荧光缀合物，在麻醉大鼠的大脑皮层中观察到过氧化氢灌注（0.03%，0.3%，流速为1 μ l/min）后丙二醛的产生显著增加。
A method for in vivo evaluation of lipid peroxidation in the extracellular space of anaesthetized rat brain cortex was developed, This method involved the use of microdialysis perfusion and high-performance liquid chromatography, The microdialysates, eluted from implanted probes, were reacted with thiobarbituric acid (TBA) prior to analysis by an HPLC system equipped with a fluorescence detector (excitation and emission wavelengths were 515 and 550 nm, respectively), Lipid peroxidation in the extracellular space was evaluated as the concentration of malondialdehyde, a lipid peroxidation end product which reacts with TBA to form a fluorescent conjugate, Significantly increased production of malondialdehyde following hydrogen peroxide perfusion (0.03%, 0.3% at a flow-rate of 1 mu l/min) was observed in the brain cortex of anaesthetized rats.