Pseudomonas aeruginosa exhibits sliding motility in the absence of type IV pili and flagella
Pseudomonas aeruginosa exhibits sliding motility in the absence of type IV pili and flagella
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DOI：
10.1128/jb.01620-07
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发表时间：
2008-04-01
影响因子：
3.2
通讯作者：
Kazmierczak, Barbara I.
中科院分区：
文献类型：
--
作者：
Murray, Thomas S.;Kazmierczak, Barbara I.
铜绿假单胞菌在特定碳源和氮源存在下，在0.5 - 1%琼脂平板上表现出群集运动。我们发现PAO 1双突变体既不表达鞭毛也不表达IV型皮利（fliC pilA），在相同条件下显示滑动运动。滑动运动被抑制时，IV型菌毛表达恢复，群集运动一样，它也减少了在没有鼠李糖脂表面活性剂的生产。转座子插入gacA和gacS增加滑动运动和恢复卷须形成蔓延的殖民地，而转座子插入retS废除运动。这些运动的变化并不伴随着可检测到的变化，鼠李糖脂表面活性剂的生产或出现的细菌表面结构，可能权力滑动运动。我们认为铜绿假单胞菌在群集过程中需要鞭毛来克服IV型皮利介导的粘附相互作用。滑动运动对环境线索和调节途径的明显依赖性也影响群集运动，这表明这两种形式的运动受到类似的内聚因素的影响，限制易位，以及分散的因素，促进传播。滑动运动的研究可能特别适合于鉴定除皮利和鞭毛之外的影响铜绿假单胞菌群落行为的因素。
Pseudomonas aeruginosa exhibits swarming motility on 0.5 to 1% agar plates in the presence of specific carbon and nitrogen sources. We have found that PAO1 double mutants expressing neither flagella nor type IV pili (fliC pilA) display sliding motility under the same conditions. Sliding motility was inhibited when type IV pilus expression was restored; like swarming motility, it also decreased in the absence of rhamnolipid surfactant production. Transposon insertions in gacA and gacS increased sliding motility and restored tendril formation to spreading colonies, while transposon insertions in retS abolished motility. These changes in motility were not accompanied by detectable changes in rhamnolipid surfactant production or by the appearance of bacterial surface structures that might power sliding motility. We propose that P. aeruginosa requires flagella during swarming to overcome adhesive interactions mediated by type IV pili. The apparent dependence of sliding motility on environmental cues and regulatory pathways that also affect swarming motility suggests that both forms of motility are influenced by similar cohesive factors that restrict translocation, as well as by dispersive factors that facilitate spreading. Studies of sliding motility may be particularly well-suited for identifying factors other than pili and flagella that affect community behaviors of P. aeruginosa.