AINTEGUMENTA-LIKE6 can functionally replace AINTEGUMENTA but alters Arabidopsis flower development when misexpressed at high levels
AINTEGUMENTA-LIKE6 can functionally replace AINTEGUMENTA but alters Arabidopsis flower development when misexpressed at high levels
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DOI：
10.1007/s11103-016-0535-y
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发表时间：
2016-11-01
影响因子：
5.1
通讯作者：
Krizek, Beth A.
中科院分区：
文献类型：
--
作者：
Han, Han;Krizek, Beth A.
表达差异是两个相关转录因子AINTEGUMENTA和AINTEGUMENTA-LIKE6之间功能差异的基础。AINTEGUMENTA-LIKE6的高水平异位表达改变了花器官的起始、生长和特性。AINTEGUMENTA(ANT)和AINTEGUMENTA-LIKE6(AIL6)编码在拟南芥花器官发育过程中部分重叠的相关转录因子。为了研究ANT和AIL6之间的功能差异是否是基因表达和/或蛋白质活性差异的结果，我们制作了转基因植物，其中AIL6的基因组拷贝在ANT启动子的控制下表达。蚂蚁：当AIL6在野生型中的表达水平与蚂蚁相似时，gAIL6可以挽救蚂蚁突变体的花器官大小缺陷。因此，ANT和AIL6之间的功能差异主要是由于基因表达的差异。然而，在较高水平表达AIL6的品系表现出额外的表型，包括花器官数量减少和花器产生马赛克。在两个不同的可诱导AIL6转基因株系中也观察到了这些表型，但在35S：ANT中没有观察到，这表明AIL6蛋白可能具有与ANT不同的活性，尽管这种差异在体内的相关性尚不清楚。与35S：蚂蚁植物类似，在诱导系中过表达AIL6也会导致更大的花的产生。在这里描述的转基因品系中由于AIL6的错误表达而导致的不同的表型，以及先前所描述的那些特征，似乎是由于不同的AIL6表达水平和模式造成的。
Expression differences underlie the functional differences between two related transcription factors: AINTEGUMENTA and AINTEGUMENTA-LIKE6. Ectopic expression of AINTEGUMENTA-LIKE6 at high levels alters floral organ initiation, growth and identity specification.AINTEGUMENTA (ANT) and AINTEGUMENTA-LIKE6 (AIL6) encode related transcription factors with partially overlapping roles in floral organ development in Arabidopsis thaliana. To investigate whether the functional differences between ANT and AIL6 are a consequence of differences in gene expression and/or protein activity, we made transgenic plants in which a genomic copy of AIL6 was expressed under the control of the ANT promoter. ANT:gAIL6 can rescue the floral organ size defects of ant mutants when AIL6 is expressed at similar levels as ANT in wild type. Thus, the functional differences between ANT and AIL6 result primarily from gene expression differences. However, lines that express AIL6 at higher levels display additional phenotypes that include reduced numbers of floral organs and the production of mosaic floral organs. These phenotypes were also observed in two different inducible AIL6 transgenic lines but not in 35S:ANT, suggesting that AIL6 protein may have activities distinct from ANT, although the in vivo relevance of such differences is not clear. Similar to 35S:ANT plants, overexpression of AIL6 in the inducible lines also results in the production of larger flowers. The distinct phenotypes resulting from AIL6 misexpression in the transgenic lines described here and those previously characterized appear to result from different levels and patterns of AIL6 expression.