In vivo validation of custom-designed silicon-based microelectrode arrays for long-term neural recording and stimulation.
In vivo validation of custom-designed silicon-based microelectrode arrays for long-term neural recording and stimulation.
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DOI：
10.1109/tbme.2011.2172440
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发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
McCreery DB
中科院分区：
文献类型：
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作者：
Han M;Manoonkitiwongsa PS;Wang CX;McCreery DB
我们开发并验证了硅基神经探针，用于长期植入大脑中的神经刺激和记录。这些探针结合了深度反应离子蚀刻工艺和尖端区域的机械成型，产生了机械坚固的柄和锋利的尖端，以减少插入大脑和脊髓的力，特别是在同一阵列中具有多个柄的情况下。阵列的插入力已在体外进行了量化。五个连续长期植入的装置在 3 至 18 个月内完全发挥作用。微电极位点电镀有氧化铱，并在体外和植入成年猫脑后进行电荷注入容量测量。通过刺激耳蜗核并记录下丘中央核诱发的神经元活动来验证慢性阵列的功能。阵列的记录质量也在体内进行了量化，记录了植入后长达 566 天的神经元尖峰活动。使用免疫组织化学染色对神经元和星形胶质细胞进行组织病理学评估表明，长期植入后组织结构的变化极小。
We developed and validated silicon-based neural probes for neural stimulating and recording in long-term implantation in the brain. The probes combine the deep reactive ion etching process and mechanical shaping of their tip region, yielding a mechanically sturdy shank with a sharpened tip to reduce insertion force into the brain and spinal cord, particularly, with multiple shanks in the same array. The arrays’ insertion forces have been quantified in vitro. Five consecutive chronically-implanted devices were fully functional from 3 to 18 months. The microelectrode sites were electroplated with iridium oxide, and the charge injection capacity measurements were performed both in vitro and after implantation in the adult feline brain. The functionality of the chronic array was validated by stimulating in the cochlear nucleus and recording the evoked neuronal activity in the central nucleus of the inferior colliculus. The arrays’ recording quality has also been quantified in vivo with neuronal spike activity recorded up to 566 days after implantation. Histopathology evaluation of neurons and astrocytes using immunohistochemical stains indicated minimal alterations of tissue architecture after chronic implantation.