Plasma minichromosome maintenance complex component 6 is a novel biomarker for hepatocellular carcinoma patients
Plasma minichromosome maintenance complex component 6 is a novel biomarker for hepatocellular carcinoma patients
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DOI：
10.1111/hepr.12303
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发表时间：
2014-12-01
影响因子：
4.2
通讯作者：
Yang, Yuxiu
中科院分区：
文献类型：
--
作者：
Zheng, Tenghao;Chen, Ming;Yang, Yuxiu
目的探讨血浆微小染色体维持复合物6（minichromosome maintenance complex component 6，MCM6）mRNA和蛋白在肝细胞癌（hepatocellular carcinoma，HCC）患者中的表达及其对HCC的诊断价值。从所有样品的血浆中提取循环RNA。通过实时聚合酶链反应扩增并定量MCM6的mRNA。血浆MCM6和甲胎蛋白（AFP）的蛋白水平测定酶联免疫吸附试验。结果在肝癌患者，MCM6的mRNA和蛋白水平显着增加，在肝硬化和健康对照组。HCC患者血浆中MCM6 mRNA和蛋白水平与血管浸润程度相关（P
AimThis study aimed to investigate the presence of plasma minichromosome maintenance complex component 6 (MCM6) mRNA and protein levels in hepatocellular carcinoma (HCC) patients and evaluate their diagnostic value for HCC.MethodsBlood samples were collected from 61 HCC and 29 cirrhotic patients, and 30 healthy individuals. Circulating RNA was extracted from plasma of all samples. The mRNA for MCM6 were amplified and quantified by real-time polymerase chain reaction. Plasma MCM6 and -fetoprotein (AFP) protein levels were measured by enzyme-linked immunosorbent assay.ResultsIn HCC patients, MCM6 mRNA and protein levels were significantly increased over the cirrhotic and healthy controls. The levels of MCM6 mRNA and protein in the plasma of HCC patients correlated to vascular invasion (P