SEQUENCE AND GENOMIC ORGANIZATION OF THE HSP70 GENES OF LEISHMANIA-AMAZONENSIS
SEQUENCE AND GENOMIC ORGANIZATION OF THE HSP70 GENES OF LEISHMANIA-AMAZONENSIS
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DOI：
10.1016/0166-6851(93)90108-a
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发表时间：
1993-12-01
影响因子：
1.5
通讯作者：
LANGER, PJ
中科院分区：
文献类型：
--
作者：
BOCK, JH;LANGER, PJ
对亚马逊利什曼原虫hsp 70基因的序列和基因组结构进行了研究。重叠粘粒克隆图谱显示7个L.亚马逊热休克蛋白70基因组成一个24-kb的基因座，含有3.5-kb的串联重复序列。覆盖不同的染色体区域的Cosmetic表明，第八hsp 70序列位于一个遥远的网站。Southern杂交结果表明，存在额外的热休克蛋白70基因或假基因。一个完整的3.5 kb的基因组重复单位，包括编码区和基因间区，进行测序。预测的L.亚马孙人HSP 70蛋白与杜氏利什曼原虫或硕大利什曼原虫HSP 70具有约95%的序列同一性，与锥虫HSP 70具有81-85%的同一性，与人HSP 70或HSP 70同源物分别具有68%或72%的同一性。在其他锥虫HSP 70蛋白中发现的GGMP四肽废除在L.亚马逊层序L. amazonensis和L. major的差异主要表现在L.亚马逊层序在上游序列中确定了潜在的调节热休克元件。还分离了几个cDNA克隆，并鉴定了两个相距100个核苷酸的不同聚（Af）添加位点。
The sequence and genomic organization of hsp70 genes in Leishmania amazonensis were examined. Maps of overlapping cosmid clones revealed that seven L. amazonensis hsp70 genes are organized into a 24-kb locus containing 3.5-kb tandem repeats. Cosmids covering a different chromosomal region indicated that an eighth hsp70 sequence is located at a distant site. Southern blot data suggested the existence of additional hsp70 genes or pseudogenes. One complete 3.5-kb genomic repeat unit, including coding and intergenic regions, was sequenced. The predicted L. amazonensis HSP70 protein had approximately 95% sequence identity with Leishmania donovani or Leishmania major HSP70, 81-85% identity with trypanosome HSP70, and 68 or 72% identity with human HSP70 or HSP70 cognate, respectively. The GGMP tetrapeptide repeal found in other trypanosomatid HSP70 proteins is absent from the L. amazonensis sequence. Intergenic sequences of L. amazonensis and L. major differed mainly in the presence of short gaps in the L. amazonensis sequence. Potential regulatory heat shock elements were identified in the upstream sequence. Several cDNA clones were also isolated, and two different poly(Af addition sites 100 nucleotides apart were identified.