Responses of the pea (Pisum sativum L.) leaf metabolome to drought stress assessed by nuclear magnetic resonance spectroscopy
Responses of the pea (Pisum sativum L.) leaf metabolome to drought stress assessed by nuclear magnetic resonance spectroscopy
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DOI：
10.1007/s11306-008-0128-0
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发表时间：
2008-12-01
期刊：
影响因子：
3.6
通讯作者：
Domoney, Claire
中科院分区：
文献类型：
--
作者：
Charlton, Adrian J.;Donarski, James A.;Domoney, Claire
虽然许多化合物在实验室干旱胁迫实验中发生了变化，但人们对这些化合物在田间条件下的变化和重要性知之甚少。豌豆（Pisum sativum L.）(pea)叶代谢组已被剖析，使用1D和2D NMR光谱，监测干旱胁迫引起的变化，在温室和模拟田间条件下。共振的显着变化归因于一系列化合物，确定为初级和次级代谢产物，突出强调应激反应的代谢途径。重要的是，这些影响在具有高度不同条件的不同实验中基本一致。在所有生长条件下，在干旱胁迫植物中以显著较高浓度存在的代谢物包括脯氨酸、缬氨酸、苏氨酸、高丝氨酸、肌醇、γ-氨基丁酸（GABA）和胡芦巴碱（烟酸甜菜碱）。在不同的实验中，代谢物的相对量发生了变化，但并不特别与干旱胁迫相关，也被确定。这些包括谷氨酸、天冬酰胺和苹果酸，最后一种在田间实验中生长的植物中的浓度高出5倍。预计这些变化可能会对植物性能和作物最终用途产生影响。
While many compounds have been reported to change in laboratory based drought-stress experiments, little is known about how such compounds change, and are significant, under field conditions. The Pisum sativum L. (pea) leaf metabolome has been profiled, using 1D and 2D NMR spectroscopy, to monitor the changes induced by drought-stress, under both glasshouse and simulated field conditions. Significant changes in resonances were attributed to a range of compounds, identified as both primary and secondary metabolites, highlighting metabolic pathways that are stress-responsive. Importantly, these effects were largely consistent among different experiments with highly diverse conditions. The metabolites that were present at significantly higher concentrations in drought-stressed plants under all growth conditions included proline, valine, threonine, homoserine, myoinositol, gamma-aminobutyrate (GABA) and trigonelline (nicotinic acid betaine). Metabolites that were altered in relative amounts in different experiments, but not specifically associated with drought-stress, were also identified. These included glutamate, asparagine and malate, with the last being present at up to 5-fold higher concentrations in plants grown in field experiments. Such changes may be expected to impact both on plant performance and crop end-use.