INCREASED GLYCOLYTIC METABOLISM IN CARDIAC HYPERTROPHY AND CONGESTIVE FAILURE
INCREASED GLYCOLYTIC METABOLISM IN CARDIAC HYPERTROPHY AND CONGESTIVE FAILURE
复制标题
DOI：
10.1152/ajplegacy.1970.218.1.153
复制
发表时间：
1970-01-01
影响因子：
--
通讯作者：
ALTSCHULD, RA
中科院分区：
文献类型：
--
作者：
BISHOP, SP;ALTSCHULD, RA
方法动物制备。体重14-23 kg的健康1 - 3岁犬用于实验程序。给动物喂食谷物型狗粮（Ken-L-Ration，Quaker Oats Company），在实验过程中，除了与腹水发生相关的体重增加外，其体重未发生明显变化。在11只狗的主肺动脉周围通过外科手术植入橡胶Jacobson袖带，造成进行性肺动脉狭窄（6）。在甲氧氟烷麻醉下，通过经皮注射液体至位于皮下的连接球，以每两周一次的间隔使肺动脉逐渐变窄。在此过程中，使用连接到斯坦森P23 AA传感器的E-规格聚乙烯导管监测右心室压力，并显示在直写式心电图上。右心室的大小逐渐增大，如胸部X线片和电影心血管造影片上心脏大小和壁厚的定性增加、心电图上右心室肥大（RVH）模式的出现（13）以及尸检时确定的右心室质量增加所确定。实验在手术后55-80天终止，此时狗处于晚期充血性心力衰竭，如通过以下体征的存在所确定的：静脉扩张、可触知的肝脏增大、腹水、观察到的运动耐量降低和右心室舒张末期压升高超过7 mm Hg。对照组由9只未进行胸部手术的正常犬和4只在6-10周前接受假开胸术和肺动脉剥离术的犬组成。由于未手术和假手术动物之间未发现功能或生化差异，因此将其视为一组。第三组由8只继发于犬心丝虫感染的自发获得性RVH和CHF犬组成。该组动物的心肺功能不全体征持续2个月至2年以上。通过电影心血管造影显示典型的肺动脉扩张，心电图显示右心室肥大模式（13），肺动脉和右心室收缩压和舒张末期压升高，存在D。外周血中存在犬细小丝虫，尸检时右心室和肺动脉中存在成虫寄生虫，
METHODSAnimal preparation. Healthy l-to 3-year-old dogs weighing 14-23 kg were used for experimental procedures. The animals were fed a cereal type dog food (Ken-L-Ration, Quaker Oats Company), and their weight did not change appre-ciably during the course of the experiment except for an increase associated with the development of ascites. Progressive pulmonary artery stenosis was produced in 11 dogs by means of a rubber Jacobson cuff surgically implanted around the main pulmonary artery (6). With the animal under methoxyflurane anesthesia, the pulmonary artery was progressively narrowed at biweekly intervals by percutaneous injection of fluid into a connecting bulb located subcutaneously. Right ventricular pressure was monitored during this procedure using a E-gauge poly-ethylene catheter attached to a Statham P23AA transducer and displayed on a direct-writing oscillograph. The right ventricle progressively increased in size, as determined by a qualitative increase in heart size and wall thickness on thoracic radiographs and cineangiocardiograms, the appearance of a right ventricular hypertrophy(RVH) pattern on the electrocardiogram(13), and increased right ventricular mass determined at necropsy. The experiment was terminated 55-80 days following surgery, when the dogs were in advanced congestive heart failure as determined by the presence of the folIowing signs: venous distention, palpable liver enlargement, ascites, an observed decrease in exercise tolerance, and an elevation of right ventricular end-diastolic pressure above 7 mm Hg. The control group consisted of nine normal dogs without thoracic surgery and four dogs that had undergone a sham thoracotomy and pulmonary artery dissection 6-10 weeks previously. Since no functional or biochemical differences were found between the unoperated and sham-operated animals, they were considered as one group. The third group consisted of eight dogs with spontaneously acquired RVH and CHF secondary to infestation with the canine heartworm Dirofdaria immitis. Animals in this group had signs of cardiopulmonary insufficiency for from 2 months to over 2 years. The diagnosis was confirmed by the demonstration of typically dilated pulmonary arteries by cineangiocardiogram, a right ventricular hypertrophy pattern on the electrocardiogram(13), elevated tiean pulmonary artery and right ventricular systolic and enddiastolic pressure, presence of D. immitis microfilaria in the peripheral blood, presence of adult parasites in the right ventricle and pulmonary artery at necropsy, and micro-