Sequence analysis and characterization of sulfonamide resistance plasmid pRF-1 from Salmonella enterica serovar Choleraesuis
Sequence analysis and characterization of sulfonamide resistance plasmid pRF-1 from Salmonella enterica serovar Choleraesuis
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DOI：
10.1016/j.plasmid.2004.07.002
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发表时间：
2004-11-01
期刊：
影响因子：
2.6
通讯作者：
Danbara, H
中科院分区：
文献类型：
--
作者：
Haneda, T;Okada, N;Danbara, H
测定了从猪霍乱沙门氏菌分离的小质粒pRF-1的核苷酸序列。我们鉴定了7个开放阅读框（ORF），共编码6066个核苷酸，总G + C含量为53.6%。完整核苷酸序列的分析揭示了pRF-1的复制子与p15 A复制起点具有高度相似性，并可能具有cer-like区域。ORF 1是由。的816个核苷酸，显示出高度的相似性，二氢蝶酸合成酶编码的sulII基因从质粒在几个肠道致病性细菌，其功能作为磺胺类药物抗性决定簇。事实上，携带pRF-1的沙门氏菌和大肠杆菌菌株被发现对磺胺噻唑表现出很强的耐药性，这表明orf 1是一个功能基因。7个ORF中有4个编码功能未知的蛋白质。(C)2004年爱思唯尔公司All rights reserved.
The nucleotide sequence of a small plasmid, designated pRF-1, isolated from Salmonella enterica serovar Choleraesuis, was determined. We identified seven open reading frames (ORFs) encoded by 6066 nucleotides with a total G + C content of 53.6%. Analysis of the complete nucleotide sequence revealed a replicon of pRF-l to have high similarity to the p15A origin of replication, with a possible cer-like region. ORF1, which is composed. of 816 nucleotides, shows a high degree of similarity to dihydropteroate synthetase encoded by the sulII gene from plasmids in several enteropathogenic bacteria, which functions as the sulfonamide resistance determinant. In fact, Salmonella and Escherichia coli strains carrying pRF-1 were found to show strong resistance to sulfathiazole, suggesting that orf1 is a functional gene. Four of seven ORFs were found to encode putative proteins of unknown function. (C) 2004 Elsevier Inc. All rights reserved.