Comparison of Bruker Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometer to BD Phoenix Automated Microbiology System for Identification of Gram-Negative Bacilli
Comparison of Bruker Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometer to BD Phoenix Automated Microbiology System for Identification of Gram-Negative Bacilli
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DOI：
10.1128/jcm.01890-10
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发表时间：
2011-03-01
影响因子：
9.4
通讯作者：
Patel, Robin
中科院分区：
文献类型：
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作者：
Saffert, Ryan T.;Cunningham, Scott A.;Patel, Robin
我们比较了BD Phoenix自动微生物学系统与Bruker Biotyper（版本2.0）基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱（MS）系统用于鉴定革兰氏阴性杆菌，使用生化检测和/或基因测序来解决不一致的结果。BD Phoenix分别在属和种水平上正确鉴定了363株（83%）和330株（75%）分离株。布鲁克生物分型仪分别在属和种水平上正确鉴定了408株（93%）和360株（82%）分离株。将440株分离株分为临床实验室常见（308株）和罕见（132株）分离株。对于308个常见分离株，BD Phoenix和Bruker Biotyper分别正确鉴定了294个（95%）和296个（96%）属水平的分离株。对于物种鉴定，BD Phoenix和Bruker Biotyper正确鉴定了93%的常见分离株（分别为285和286）。相比之下，对于132个罕见分离株，Bruker Biotyper分别正确鉴定了112个（85%）和74个（56%）分离株的属和种水平，而BD Phoenix分别仅鉴定了69个（52%）和45个（34%）分离株的属和种水平。从统计学角度来看，Bruker Biotyper在该样本集中鉴定革兰氏阴性杆菌的属（P < 0.0001）和种（P = 0.0005）水平上总体优于BD Phoenix。在常见和少见两种类型中，两种仪器对常见革兰阴性杆菌的鉴定结果差异无统计学意义（P > 0.05）。然而，Bruker Biotyper在鉴定罕见分离的革兰氏阴性杆菌方面优于BD Phoenix（P < 0.0001）。
We compared the BD Phoenix automated microbiology system to the Bruker Biotyper (version 2.0) matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) system for identification of Gram-negative bacilli, using biochemical testing and/or genetic sequencing to resolve discordant results. The BD Phoenix correctly identified 363 (83%) and 330 (75%) isolates to the genus and species level, respectively. The Bruker Biotyper correctly identified 408 (93%) and 360 (82%) isolates to the genus and species level, respectively. The 440 isolates were grouped into common (308) and infrequent (132) isolates in the clinical laboratory. For the 308 common isolates, the BD Phoenix and Bruker Biotyper correctly identified 294 (95%) and 296 (96%) of the isolates to the genus level, respectively. For species identification, the BD Phoenix and Bruker Biotyper correctly identified 93% of the common isolates (285 and 286, respectively). In contrast, for the 132 infrequent isolates, the Bruker Biotyper correctly identified 112 (85%) and 74 (56%) isolates to the genus and species level, respectively, compared to the BD Phoenix, which identified only 69 (52%) and 45 (34%) isolates to the genus and species level, respectively. Statistically, the Bruker Biotyper overall outperformed the BD Phoenix for identification of Gram-negative bacilli to the genus (P < 0.0001) and species (P = 0.0005) level in this sample set. When isolates were categorized as common or infrequent isolates, there was statistically no difference between the instruments for identification of common Gram-negative bacilli (P > 0.05). However, the Bruker Biotyper outperformed the BD Phoenix for identification of infrequently isolated Gram-negative bacilli (P < 0.0001).