Developing a peptide-based near-infrared molecular probe for protease sensing
Developing a peptide-based near-infrared molecular probe for protease sensing
复制标题
DOI：
10.1021/bc049924s
复制
发表时间：
2004-11-01
影响因子：
4.7
通讯作者：
Tung, CH
中科院分区：
文献类型：
--
作者：
Pham, W;Choi, YD;Tung, CH
近年来，近红外（NIR）分子探针已成为多种生物医学成像的重要报告分子。设计了一种基于肽的近红外荧光探针，该探针由近红外荧光发射器（Cy5.5）、近红外荧光吸收器（NIRQ820）和蛋白酶选择性肽序列组成，用于检测蛋白酶活性。利用MMP-7模型，我们发现NIRQ820有效地吸收了Cy5.5的发射能量，导致了较低的初始信号。在与靶蛋白MMP-7反应后，所设计探针的荧光信号增加了7倍，K-cat/ K-m为10 000 M-1 s（-1）。该合成策略在其他近红外探针制备中具有广泛的应用前景。
Recently near-infrared (NIR) molecular probes have become important reporter molecules for a number of types of in vivo biomedical imaging. A peptide-based NIR fluorescence probe consisting of a NIR fluorescence emitter (Cy5.5), a NIR fluorescence absorber (NIRQ820), and a protease selective peptide sequence was designed to sense protease activity. Using a MMP-7 model, we showed that NIRQ820 efficiently absorbs the emission energy of Cy5.5 resulting in a low initial signal. Upon reacting with its target, MMP-7, the fluorescence signal of the designed probe was increased by 7-fold with a K-cat/ K-m of 100 000 M-1 s(-1). The described synthetic strategy should have wide application for other NIR probe preparations.