Identity of the heme-not-containing protein in bovine heart cytochrome c1 preparation with the protein mediating c1-c complex formation--a protein with high glutamic acid content.
Identity of the heme-not-containing protein in bovine heart cytochrome c1 preparation with the protein mediating c1-c complex formation--a protein with high glutamic acid content.
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牛心细胞色素 c1 制剂中不含血红素的蛋白质与介导 c1-c 复合物形成的蛋白质（一种高谷氨酸含量的蛋白质）的同一性。
DOI：
10.1093/oxfordjournals.jbchem.a133901
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发表时间：
1982
影响因子：
2.7
通讯作者：
King,TE
中科院分区：
文献类型：
--
作者：
Wakabayashi,S;Takeda,H;Matsubara,H;Kim,CH;King,TE
从细胞色素1制备液中分离得到一个不含血红素的蛋白质。通过胰蛋白酶和胰凝乳蛋白酶肽的分析以及固相序列分析确定了该蛋白质的氨基酸序列。它由78个氨基酸残基组成，分子量计算为9，175。该蛋白质含有高比例的谷氨酸和谷氨酰胺（占总残基的27%），但不含甲硫氨酸、异亮氨酸、酪氨酸和色氨酸。最显著的特征是在氨基（N）-末端附近的8个连续谷氨酸残基的酸性簇。二级结构被预测有一个高比例的螺旋content.The氨基酸组成和N-末端序列的蛋白质独立制备的牛心脏线粒体，这是必不可少的cytochromec 1-ccomplex的形成，这表明，这种无色的因素和本血红素不含蛋白质是相同的。有证据表明，另一种蛋白质，称为非血红素蛋白，分离自“双带”细胞色素制备，也是相同的蛋白质，本文提出的。
A heme-not-containing protein was isolated from cytochromec1preparation by gel filtration after carboxymethylation and citraconylation. The amino acid sequence of this protein was determined by the analyses of tryptic and chymotryptic peptides as well as by solid-phase sequence analysis. It consisted of 78 amino acid residues and the molecular weight was calculated to be 9,175. This protein contained a high proportion of glutamic acid and glutamine (27 % of the total residues) but no methionine, isoleucine, tyrosine, and tryptophan. The most notable feature was an acidic cluster of 8 consecutive glutamic acid residues near the amino(N)-terminus. The secondary structure was predicted to have a high proportion of helical content.The amino acid composition and N-terminal sequence of a protein independently prepared from bovine heart mitochondria, which is essential to the formation of the cytochromec1-ccomplex, suggested that this colorless factor and the present heme-not-containing protein are identical. Evidence shows that another protein, called the non-heme protein, isolated from “two-band” cytochromec1preparation is also the same protein as that presented in this paper.