SWI/SNF is required for transcriptional memory at the yeast GAL gene cluster
SWI/SNF is required for transcriptional memory at the yeast GAL gene cluster
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DOI：
10.1101/gad.1506607
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发表时间：
2007-04-15
影响因子：
10.5
通讯作者：
Peterson, Craig L.
中科院分区：
文献类型：
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作者：
Kundu, Sharmistha;Horn, Peter J.;Peterson, Craig L.
核小体组蛋白的翻译后修饰被认为有助于表观遗传转录记忆。我们描述了在酵母中的转录记忆的情况下，GAL 1的转录诱导率由先前的表达状态调节。这种表观遗传状态由子细胞遗传，但不需要组蛋白乙酰转移酶Gcn 5 p、组蛋白泛素化酶Rad 6p或组蛋白甲基化酶Dot 1 p、Set 1 p或Set 2 p。相反，我们发现，ATP依赖性染色质重塑酶，SWI/SNF，是必不可少的转录记忆在GAL 1。遗传研究表明，SWI/SNF通过拮抗ISWI样染色质重塑酶来控制转录记忆。
Post-translational modification of nucleosomal histones has been suggested to contribute to epigenetic transcriptional memory. We describe a case of transcriptional memory in yeast where the rate of transcriptional induction of GAL1 is regulated by the prior expression state. This epigenetic state is inherited by daughter cells, but does not require the histone acetyltransferase, Gcn5p, the histone ubiquitinylating enzyme, Rad6p, or the histone methylases, Dot1p, Set1p, or Set2p. In contrast, we show that the ATP-dependent chromatin remodeling enzyme, SWI/SNF, is essential for transcriptional memory at GAL1. Genetic studies indicate that SWI/SNF controls transcriptional memory by antagonizing ISWI-like chromatin remodeling enzymes.