Generation of an immortalized mouse embryonic palatal mesenchyme cell line.
Generation of an immortalized mouse embryonic palatal mesenchyme cell line.
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DOI：
10.1371/journal.pone.0179078
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Soriano P
中科院分区：
文献类型：
--
作者：
Fantauzzo KA;Soriano P
腭发育是一个复杂的形态发生过程，其中断导致人类中高度流行的出生缺陷。近几十年来，使用模型系统，如转基因小鼠，小鼠腭器官培养和原代小鼠胚胎腭间充质（MEPM）文化提供了显着的洞察腭裂的分子和细胞缺陷。然而，这些系统中的每一个的缺点已经阻止了体外腭发育的高通量、大规模研究。在这里，我们报告的永生化MEPM细胞系，保持原代MEPM细胞的形态，迁移能力，转录表达和对外源性生长因子的反应，与原代培养物的增殖潜力增加的一代。在这项研究中描述的永生化方法将促进腭间充质细胞的产生，从一个单一的基因修饰的小鼠胚胎具有无限的扩展能力，并使腭发育的机制研究，还没有可能使用原代培养。
Palatogenesis is a complex morphogenetic process, disruptions in which result in highly prevalent birth defects in humans. In recent decades, the use of model systems such as genetically-modified mice, mouse palatal organ cultures and primary mouse embryonic palatal mesenchyme (MEPM) cultures has provided significant insight into the molecular and cellular defects underlying cleft palate. However, drawbacks in each of these systems have prevented high-throughput, large-scale studies of palatogenesis in vitro. Here, we report the generation of an immortalized MEPM cell line that maintains the morphology, migration ability, transcript expression and responsiveness to exogenous growth factors of primary MEPM cells, with increased proliferative potential over primary cultures. The immortalization method described in this study will facilitate the generation of palatal mesenchyme cells with an unlimited capacity for expansion from a single genetically-modified mouse embryo and enable mechanistic studies of palatogenesis that have not been possible using primary culture.